BRENDA - Enzyme Database
show all sequences of 3.1.1.65

Eukaryotic and bacterial gene clusters related to an alternative pathway of non-phosphorylated L-rhamnose metabolism

Watanabe, S.; Saimura, M.; Makino, K.; J. Biol. Chem. 283, 20372-20382 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Azotobacter vinelandii
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Debaryomyces hansenii
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Scheffersomyces stipitis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-rhamnono-1,4-lactone + H2O
Azotobacter vinelandii
-
L-rhamnonate
-
-
?
L-rhamnono-1,4-lactone + H2O
Debaryomyces hansenii
-
L-rhamnonate
-
-
?
L-rhamnono-1,4-lactone + H2O
Scheffersomyces stipitis
-
L-rhamnonate
-
-
?
L-rhamnono-1,4-lactone + H2O
Debaryomyces hansenii NBRC0083
-
L-rhamnonate
-
-
?
L-rhamnono-1,4-lactone + H2O
Azotobacter vinelandii NBRC 102612
-
L-rhamnonate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Azotobacter vinelandii
-
-
-
Azotobacter vinelandii NBRC 102612
-
-
-
Debaryomyces hansenii
-
-
-
Debaryomyces hansenii NBRC0083
-
-
-
Scheffersomyces stipitis
A3LZU8
-
-
Purification (Commentary)
Purification (Commentary)
Organism
nickel-nitrilotriacetic acid superflow column
Azotobacter vinelandii
nickel-nitrilotriacetic acid superflow column
Debaryomyces hansenii
nickel-nitrilotriacetic acid superflow column
Scheffersomyces stipitis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-rhamnono-1,4-lactone + H2O
-
687737
Azotobacter vinelandii
L-rhamnonate
-
-
-
?
L-rhamnono-1,4-lactone + H2O
-
687737
Debaryomyces hansenii
L-rhamnonate
-
-
-
?
L-rhamnono-1,4-lactone + H2O
-
687737
Scheffersomyces stipitis
L-rhamnonate
-
-
-
?
L-rhamnono-1,4-lactone + H2O
-
687737
Debaryomyces hansenii NBRC0083
L-rhamnonate
-
-
-
?
L-rhamnono-1,4-lactone + H2O
-
687737
Azotobacter vinelandii NBRC 102612
L-rhamnonate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
L-rhamnono-gamma-lactonase
-
Azotobacter vinelandii
L-rhamnono-gamma-lactonase
-
Debaryomyces hansenii
L-rhamnono-gamma-lactonase
-
Scheffersomyces stipitis
LRA2
-
Azotobacter vinelandii
LRA2
-
Scheffersomyces stipitis
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Azotobacter vinelandii
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Debaryomyces hansenii
overexpression in Escherichia coli DH5 alpha as His6-tagged enzyme
Scheffersomyces stipitis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
L-rhamnono-1,4-lactone + H2O
Azotobacter vinelandii
-
L-rhamnonate
-
-
?
L-rhamnono-1,4-lactone + H2O
Debaryomyces hansenii
-
L-rhamnonate
-
-
?
L-rhamnono-1,4-lactone + H2O
Scheffersomyces stipitis
-
L-rhamnonate
-
-
?
L-rhamnono-1,4-lactone + H2O
Debaryomyces hansenii NBRC0083
-
L-rhamnonate
-
-
?
L-rhamnono-1,4-lactone + H2O
Azotobacter vinelandii NBRC 102612
-
L-rhamnonate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
nickel-nitrilotriacetic acid superflow column
Azotobacter vinelandii
nickel-nitrilotriacetic acid superflow column
Debaryomyces hansenii
nickel-nitrilotriacetic acid superflow column
Scheffersomyces stipitis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-rhamnono-1,4-lactone + H2O
-
687737
Azotobacter vinelandii
L-rhamnonate
-
-
-
?
L-rhamnono-1,4-lactone + H2O
-
687737
Debaryomyces hansenii
L-rhamnonate
-
-
-
?
L-rhamnono-1,4-lactone + H2O
-
687737
Scheffersomyces stipitis
L-rhamnonate
-
-
-
?
L-rhamnono-1,4-lactone + H2O
-
687737
Debaryomyces hansenii NBRC0083
L-rhamnonate
-
-
-
?
L-rhamnono-1,4-lactone + H2O
-
687737
Azotobacter vinelandii NBRC 102612
L-rhamnonate
-
-
-
?
Other publictions for EC 3.1.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697897
Watanabe
Novel modified version of nonp ...
Sphingomonas sp.
FEBS J.
276
1554-1567
2009
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687737
Watanabe
Eukaryotic and bacterial gene ...
Azotobacter vinelandii, Azotobacter vinelandii NBRC 102612, Debaryomyces hansenii, Debaryomyces hansenii NBRC0083, Scheffersomyces stipitis
J. Biol. Chem.
283
20372-20382
2008
-
-
3
-
-
-
-
-
-
-
-
5
-
13
-
-
3
-
-
-
-
-
5
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
3
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
347736
Twerdochlib
-
L-Rhamnose metabolism in Pichi ...
Scheffersomyces stipitis, Schwanniomyces polymorphus, Schwanniomyces polymorphus 1747
Can. J. Microbiol.
40
896-902
1994
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
-
3
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
3
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643844
Rigo
-
Oxidative pathway for L-rhamno ...
Pullularia pullulans
Can. J. Microbiol.
31
817-822
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-