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Literature summary for 3.1.1.64 extracted from

  • Nikolaeva, O.; Takahashi, Y.; Moiseyev, G.; Ma, J.X.
    Purified RPE65 shows isomerohydrolase activity after reassociation with a phospholipid membrane (2009), FEBS J., 276, 3020-3030.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.64

Cloned(Commentary)

Cloned (Comment) Organism
expressed in 293A-LRAT cells Gallus gallus

General Stability

General Stability Organism
0.1% (w/v) CHAPS is optimal for solubilizing RPE65 while preserving its enzymatic activity Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
CHAPS the addition of 0.5% (w/v) CHAPS to the assay system almost completely abolishes the 11-cis-retinol formation Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
 all-trans-retinyl palmitate purified enzyme, in 10 mM BTP buffer, pH 8.0, 100 mM NaCl, at 37°C Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Gallus gallus 16020
-

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
retinal pigment epithelium
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
all-trans-retinyl ester + H2O all-trans-retinyl ester must be incorporated into the phospholipid membrane to serve as a substrate for isomerohydrolase RPE65 Gallus gallus 11-cis-retinol + a fatty acid
-
 ?
all-trans-retinyl palmitate + H2O high affinity substrate Gallus gallus 11-cis-retinol + palmitate
-
 ?
additional information neither retinyl ester nor 11-cis-retinol is produced after incubation of all-trans-retinol with the purified RPE65 (not an intrinsic substrate for RPE65) Gallus gallus ?
-
 ?

Synonyms

Synonyms Comment Organism
Rpe65
-
 Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000145
-
 all-trans-retinyl palmitate purified enzyme, in 10 mM BTP buffer, pH 8.0, 100 mM NaCl, at 37°C Gallus gallus

General Information

General Information Comment Organism
physiological function RPE65 is the isomerohydrolase of the visual cycle Gallus gallus