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Literature summary for 3.1.1.6 extracted from

  • Wu, Z.; Chen, J.; Zhang, Z.; Ma, L.; Xu, T.; Yu, H.; Zhang, Q.; Chen, Y.
    Development of Escherichia coli strain with enhanced enzymatic activity of acetyl esterase using a strategy of tandem repetitive promoters (2017), Waste Biomass Valor., 8, 2339-2348 .
No PubMed abstract available
Search for more literature for 3.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM110 cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 77.86% residual activity at 2 mM Escherichia coli
Fe3+ 77.37% residual activity at 2 mM Escherichia coli
K+ 79.8% residual activity at 2 mM Escherichia coli
Mg2+ 67.81% residual activity at 2 mM Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 106.21% activity at 0.5 mM Escherichia coli
Co2+ 105.61% activity at 0.5 mM Escherichia coli
Ni2+ 110.32% activity at 0.5 mM Escherichia coli
Zn2+ 103.63% activity at 0.5 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A0A7HLG5
-
-
Escherichia coli RB3 A0A0A7HLG5
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE cellulose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Escherichia coli 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O
-
 Escherichia coli RB3 4-nitrophenol + acetate
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
Acetyl esterase
-
 Escherichia coli
ybaC
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 51 the enzyme maintains more than 80% activity between 35 and 51°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.09
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about 35% activity at pH 7.0, 100% activity at pH 8.0, about 60% activity at pH 9.0, almost no activity below pH 6.0 and above pH 10.0 Escherichia coli