Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.6 extracted from

  • Oh, C.; Ryu, B.H.; An, D.R.; Nguyen, D.D.; Yoo, W.; Kim, T.; Ngo, T.D.; Kim, H.S.; Kim, K.K.; Kim, T.D.
    Structural and biochemical characterization of an octameric carbohydrate acetylesterase from Sinorhizobium meliloti (2016), FEBS Lett., 590, 1242-1252 .
    View publication on PubMed
Search for more literature for 3.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sinorhizobium meliloti

Crystallization (Commentary)

Crystallization (Comment) Organism
using 0.2 M ammonium phosphate (pH 4.6), and 20% (w/v) polyethylene glycol 3350 Sinorhizobium meliloti

Protein Variants

Protein Variants Comment Organism
F152A the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis Sinorhizobium meliloti
M155A the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis Sinorhizobium meliloti
N96A the mutant has almost no activity Sinorhizobium meliloti
R69A the mutant has about 25% activity for 1-naphthyl acetate hydrolysis compared to the wild type enzyme Sinorhizobium meliloti
S13A the mutant shows complete loss of activity Sinorhizobium meliloti
W151A the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis Sinorhizobium meliloti
Y101A the mutant has almost no activity Sinorhizobium meliloti

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti Q92XZ6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O highest activity (100%) Sinorhizobium meliloti 1-naphthol + acetate
-
 ?
1-naphthyl butyrate + H2O 36% activity compared to 1-naphthyl acetate Sinorhizobium meliloti 1-naphthol + butyrate
-
 ?
2-naphthyl acetate + H2O about 40% activity compared to 1-naphthyl acetate Sinorhizobium meliloti 2-naphthol + acetate
-
 ?
4-methylumbelliferyl acetate + H2O
-
 Sinorhizobium meliloti 4-methylumbelliferone + acetate
-
 ?
acetyl alginate + H2O
-
 Sinorhizobium meliloti alginate + acetate
-
 ?
cellulose acetate + H2O
-
 Sinorhizobium meliloti cellulose + acetate
-
 ?
D-glucose penta-acetate + H2O
-
 Sinorhizobium meliloti ?
-
 ?
additional information no activity with 1-naphthyl phosphate, 4-methylumbelliferyl phosphate, linalyl acetate, alpha-terpinyl acetate, glyceryl trioleate, fish oil, or olive oil Sinorhizobium meliloti ?
-
 ?
tert-butyl acetate + H2O
-
 Sinorhizobium meliloti 2-methylpropane + acetate
-
 ?

Subunits

Subunits Comment Organism
octamer
-
 Sinorhizobium meliloti

Synonyms

Synonyms Comment Organism
carbohydrate acetylesterase
-
 Sinorhizobium meliloti
Est24
-
 Sinorhizobium meliloti