Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Talaromyces pinophilus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 170 mM ammonium sulfate, 28% (w/v) PEG 4000, 5% (v/v) glycerol and 0.5% (w/v) n-octyl-beta-D-glucopyranoside | Talaromyces pinophilus |
Protein Variants | Comment | Organism |
---|---|---|
C16A | the mutant shows strongly reduced activity (to about 10%) compared to the wild type enzyme | Talaromyces pinophilus |
D179A | the mutant shows strongly reduced activity (to about 50%) compared to the wild type enzyme | Talaromyces pinophilus |
H182A | the mutant shows strongly reduced activity (to about 50%) compared to the wild type enzyme | Talaromyces pinophilus |
S10A | the mutant shows strongly reduced activity (to about 10%) compared to the wild type enzyme | Talaromyces pinophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
4-nitrophenyl acetate | wild type enzyme, at pH 5.5 and 50°C | Talaromyces pinophilus | |
1.4 | - |
4-nitrophenyl acetate | mutant enzyme D179A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
1.8 | - |
4-nitrophenyl acetate | mutant enzyme H182A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
4.7 | - |
4-nitrophenyl acetate | mutant enzyme C16A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
5.1 | - |
4-nitrophenyl acetate | mutant enzyme S10A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
7.1 | - |
4-nitrophenyl butyrate | wild type enzyme, at pH 5.5 and 50°C | Talaromyces pinophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | contains calcium | Talaromyces pinophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an acetic ester + H2O | Talaromyces pinophilus | - |
an alcohol + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces pinophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q column chromatography, HiTrap butyl column chromatography, and Superdex 200 gel filtration | Talaromyces pinophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | highest activity | Talaromyces pinophilus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Talaromyces pinophilus | 4-nitrophenol + butyrate | - |
? | |
an acetic ester + H2O | - |
Talaromyces pinophilus | an alcohol + acetate | - |
? | |
additional information | no activity with 4-nitrophenyl octanoate. The enzyme does not exhibit de-esterification activity toward an acetylated xylooligosaccharide extracted from hot-water treated eucalyptus | Talaromyces pinophilus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AE206 | - |
Talaromyces pinophilus |
carbohydrate esterase | - |
Talaromyces pinophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Talaromyces pinophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.8 | - |
4-nitrophenyl acetate | mutant enzyme S10A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
26.2 | - |
4-nitrophenyl acetate | mutant enzyme C16A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
29.8 | - |
4-nitrophenyl butyrate | wild type enzyme, at pH 5.5 and 50°C | Talaromyces pinophilus | |
36.3 | - |
4-nitrophenyl acetate | mutant enzyme D179A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
38.8 | - |
4-nitrophenyl acetate | mutant enzyme H182A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
49.2 | - |
4-nitrophenyl acetate | wild type enzyme, at pH 5.5 and 50°C | Talaromyces pinophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Talaromyces pinophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.1 | - |
4-nitrophenyl butyrate | wild type enzyme, at pH 5.5 and 50°C | Talaromyces pinophilus | |
4.2 | - |
4-nitrophenyl acetate | mutant enzyme S10A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
5.4 | - |
4-nitrophenyl acetate | mutant enzyme C16A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
20.7 | - |
4-nitrophenyl acetate | mutant enzyme H182A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
24.8 | - |
4-nitrophenyl acetate | mutant enzyme D179A, at pH 5.5 and 50°C | Talaromyces pinophilus | |
44.7 | - |
4-nitrophenyl acetate | wild type enzyme, at pH 5.5 and 50°C | Talaromyces pinophilus |