Literature summary for 3.1.1.6 extracted from

Chungool, W.; Thongkam, W.; Raweesri, P.; Thamchaipenet, A.; Pinphanichakarn, P.
Production, purification, and characterization of acetyl esterase from Streptomyces sp. PC22 and its action in cooperation with xylanolytic enzymes on xylan degradation (2008), World J. Microbiol. Biotechnol., 24, 549-556.

Search for more literature for 3.1.1.6

Application

Application	Comment	Organism
industry	cooperative action on birchwood xylan degradation in combination with Streptomyces sp. xylanase and beta xylosidase, production of biofuels utilizing hemicelluloses containing acetyl xylan, 1.16fold to 1.39fold increased sugar release	Streptomyces sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	acetyl esterase activity	Streptomyces sp.
Hg2+	strong inhibitor, 0.1 mM, acetyl esterase activity	Streptomyces sp.
additional information	10 mM ethylmethylaminopropyl carbodiimide or iodacetamide without effect on acetyl esterase activity	Streptomyces sp.
phenyl methylsulfonyl fluoride	10 mM, 50% decreased acetyl esterase activity	Streptomyces sp.
Zn2+	acetyl esterase activity	Streptomyces sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.43	-	p-nitrophenyl acetate	pH 6.5	Streptomyces sp.
7.8	-	Alpha-naphthyl acetate	pH 6.5	Streptomyces sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	10 mM Ca2+, Co2+, Mg2+ or Mn2+ without any affect on acetyl esterase activity	Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	SDS-PAGE	Streptomyces sp.
155000	-	native, gel-filtration on Sephacryl S-200 HR column	Streptomyces sp.

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp.	-	PC22	-

Purification (Commentary)

Purification (Comment)	Organism
at 4°C, precipitation from culture filtrate by 40-80% (NH4)2SO4 saturation followed by anion-exchange (DEAE column, elution with 0-1 M NaCl gradient), t-butyl hydrophobic (elution with 1.7-0 M (NH4)2SO4 gradient) and hydroxyapatite chromatography (elution with 0.02-0.5 M sodium phosphate buffer, pH 6.8), stored at -20°C after addition of 20% glycerol	Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
9.63	-	p-nitrophenyl acetate, after hydroxyapatite chromatography, pH 6.5	Streptomyces sp.
71	-	p-nitrophenyl acetate, pH 6.5	Streptomyces sp.
1027	-	alpha-naphthyl acetate, pH 6.5	Streptomyces sp.

Storage Stability

Storage Stability	Organism
stored at -20°C in 20% glycerol	Streptomyces sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetylxylan + H2O	60°C, pH 6, acetyl xylan esterase (acetate-releasing) activity on birchwood xylan	Streptomyces sp.	xylan + acetate	-	?
alpha-naphthyl acetate + H2O	15 min, 50°C, pH 6.5	Streptomyces sp.	alpha-naphthol + acetate	-	?
additional information	no activity on p-nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinofuranoside and no xylanolytic activity	Streptomyces sp.	?	-	?
p-nitrophenyl acetate + H2O	10 min, 50°C, pH 6.5	Streptomyces sp.	p-nitrophenol + acetate	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 38000, gel filtration on Sephacryl S-200 HR column	Streptomyces sp.

Synonyms

Synonyms	Comment	Organism
Acetyl esterase	-	Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Streptomyces sp.

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	65	-	Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	stable for 30 min at temperatures up to 60°C in absence of substrate	Streptomyces sp.

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	-	85% residual activity, alpha-naphthyl acetate as substrate, more active in acetate buffer than phosphate buffer	Streptomyces sp.
6.5	7	optimum activity, alpha-naphthyl acetate as substrate	Streptomyces sp.
8	-	65% residual activity, alpha-naphthyl acetate as substrate	Streptomyces sp.

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	9	stable for 30 min	Streptomyces sp.

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Release 2023.1 (January 2023)