Application | Comment | Organism |
---|---|---|
molecular biology | postulated PON (paraoxonase) family membership due to similarity to primary structure of PON2, plant strictosidine synthase and di-isopropyl fluorophosphatase, its N-terminal single transmembrane domain, a nine-exon gene structure, a six-bladed beta-propeller tertiary structure, and similar metabolic regulation of gene expression | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cDNA of 1248 bp in pGEM-T-easy for sequencing (polymorphic residues at position 65, 282, 374, in middle European population homozygosity for R282 and R347) and subcloning, cDNA coding for residues 45-416 in pDEST15 or residues 58-416 in pGEX-1gammaT (yielding glutathione transferase-tag) for expression in Escherichia coli BL21, cDNA coding for residues 16-416 in pDEST10 for baculovirus-mediated expression in insect cell lines Sf9 and HighFive with N-terminal hexa-His-tag | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
structural modelling using Cn3D 4.1 software and plant strictosidine synthase as model structure, six-bladed beta-propeller structure | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | metal-chelating reduces enzymatic activity by ca. 33% | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell membrane | heavy membrane after sucrose density gradient cell fractionation on Hep-G2 (full-length protein and 32 kDa variant) and RINm5F cells (full-length protein) | Homo sapiens | - |
- |
cytosol | 32 kDa low molecular weight variant, sucrose density gradient cell fractionation on RINm5F cells | Homo sapiens | 5829 | - |
extracellular | C-terminus of full-length protein (residues 62-416) as revealed by cell surface domain-specific FACS analyses on monocytes and Hep-G2 cells as well as cell surface domain-specific cytochemistry and confocal microscopy on liver, kidney, and pancreas, detection by ELISA in urine and supernatant of thrombin-treated Hep-G2 cells | Homo sapiens | - |
- |
additional information | not detectable by ELISA using cell surface domain-specific antibody | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
degradation product, Western blot, not detectable by cytosolic domain-specific antibody | Homo sapiens |
32000 | - |
low molecular variant, membrane associated (Hep-G2 cell) or cytosolic (RINm5F cell), Western blot | Homo sapiens |
50000 | - |
full-length protein, Western blot | Homo sapiens |
52000 | - |
high molecular variant in Hep-G2 and SH-SY5Y cells, Western blot | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | residues N160 and N196 are potential sites, in silico analysis | Homo sapiens |
additional information | residues C140, C149 and C409 with putative intra- and intermolecular disulfide bonds, in silico analysis | Homo sapiens |
Purification (Comment) | Organism |
---|---|
from bacterial lysate or inclusion bodies by Superose 6 column chromatography in presence of 6-8 M urea for antibody generation, from insect cells by FPLC-based metal affinity chromatography (250 mM imidazole) and anion-exchange chromatography on Q-sepharose column for activity measurements | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adult | ubiquitous transcript of ca. 2.4 kb, highest gene expression level in liver, placenta and heart, Northern blot | Homo sapiens | - |
artery wall | kidney, cytochemistry and confocal microscopy | Homo sapiens | - |
embryo | ubiquitous transcript of ca. 2.4 kb, highest gene expression level in liver and kidney, Northern blot | Homo sapiens | - |
endothelial cell | kidney, cytochemistry and confocal microscopy | Homo sapiens | - |
HEK-293 cell | lower expression level than in Hep-G2 cells as revealed by Northern blot and quantitative RT-PCR, serum-induced inhibition of gene expression (quantitative RT-PCR, FACS, reporter gene assay) | Homo sapiens | - |
Hep-G2 cell | strong expression, lower expression level than in liver as revealed by Northern blot and quantitative RT-PCR, cell fractionation, FACS analysis | Homo sapiens | - |
kidney | lower expression level than in liver as revealed by Northern blot and quantitative RT-PCR, Western blot, strong in glomeruli and tubular structures according to cytochemistry and confocal microscopy | Homo sapiens | - |
leukocyte | no expression in granulocytes and lymphocytes according to cell surface domain-specific FACS analyses | Homo sapiens | - |
liver | highest expression level as revealed by Northern blot and quantitative RT-PCR, Western blot, cytochemistry and confocal microscopy | Homo sapiens | - |
pancreatic islet | highest expression within pancreatic tissue at the islets of Langerhans, revealed by cytochemistry and confocal microscopy | Homo sapiens | - |
peripheral blood mononuclear cell | high expression on monocytes according to cell-surface domain-specific FACS analyses | Homo sapiens | - |
placenta | high expression level, Northern blot, quantitative RT-PCR | Homo sapiens | - |
SH-SY5Y cell | quantitative RT-PCR, Western blot | Homo sapiens | - |
urine | ELISA using cell surface domain-specific antibody | Homo sapiens | - |
vascular smooth muscle cell | kidney, cytochemistry and confocal microscopy | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-naphthyl acetate + H2O | sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8 | Homo sapiens | beta-naphthol + acetate | SDS-PAGE and in gel-zymography | ? | |
phenyl acetate + H2O | sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8, 3 min, 1 mM CaCl2 or 5 mM EDTA | Homo sapiens | phenol + acetate | spectrophotometry, absorbance at 270 nm | ? |
Synonyms | Comment | Organism |
---|---|---|
C20orf3 | - |
Homo sapiens |