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Literature summary for 3.1.1.6 extracted from

  • Qian, J.H.; Xu, J.H.
    Catalytic performance of a highly enantioselective (R)-ester hydrolase from a new isolate Acinetobacter sp. CGMCC 0789 (2004), J. Mol. Catal. B, 27, 227-232.
No PubMed abstract available
Search for more literature for 3.1.1.6

Activating Compound

Activating Compound Comment Organism Structure
benzethonium chloride i.e. (diisobutyl phenoxyethoxyethyl) dimethyl benzylammonium chloride, up to 2.5fold enhancement of activity Acinetobacter sp.
bis(2-ethylhexyl) sodium sulfosuccinate 138% of initial activity Acinetobacter sp.
bis(octadecyl) ammonium chloride 246% of initial activity Acinetobacter sp.
cetyltrimethyl ammonium bromide 184% of initial activity Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Acinetobacter sp.
Fe3+
-
 Acinetobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
32.5
-
 p-nitrophenyl acetate pH 7.0, 25°C, presence of benzethonium chloride Acinetobacter sp.
385
-
 p-nitrophenyl acetate pH 7.0, 25°C, absence of benzethonium chloride Acinetobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ stimulation Acinetobacter sp.
Ba2+ stimulation Acinetobacter sp.
Ca2+ up to 150% stimulation Acinetobacter sp.
Co2+ stimulation Acinetobacter sp.
Mg2+ stimulation Acinetobacter sp.
Mn2+ stimulation Acinetobacter sp.
Pb2+ stimulation Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 20000, SDS-PAGE Acinetobacter sp.

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Acinetobacter sp. CGMCC 0789
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.082
-
 substrate p-nitrophenyl laurate, pH 7.0, 25°C Acinetobacter sp.
0.251
-
 substrate p-nitrophenyl acetate, pH 7.0, 25°C Acinetobacter sp.
0.297
-
 substrate p-nitrophenyl decanoate, pH 7.0, 25°C Acinetobacter sp.
0.352
-
 substrate p-nitrophenyl hexanoate, pH 7.0, 25°C Acinetobacter sp.
0.369
-
 substrate p-nitrophenyl propionate, pH 7.0, 25°C Acinetobacter sp.
0.474
-
 substrate p-nitrophenyl butyrate, pH 7.0, 25°C Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride Acinetobacter sp. ?
-
 ?
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride Acinetobacter sp. CGMCC 0789 ?
-
 ?
p-nitrophenyl acetate + H2O
-
 Acinetobacter sp. p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O
-
 Acinetobacter sp. CGMCC 0789 p-nitrophenol + acetate
-
 ?
p-nitrophenyl butyrate + H2O
-
 Acinetobacter sp. p-nitrophenol + butyrate
-
 ?
p-nitrophenyl decanoate + H2O
-
 Acinetobacter sp. p-nitrophenol + decanoate
-
 ?
p-nitrophenyl hexanoate + H2O
-
 Acinetobacter sp. p-nitrophenol + hexanoate
-
 ?
p-nitrophenyl hexanoate + H2O
-
 Acinetobacter sp. CGMCC 0789 p-nitrophenol + hexanoate
-
 ?
p-nitrophenyl laurate + H2O
-
 Acinetobacter sp. p-nitrophenol + laurate
-
 ?
p-nitrophenyl laurate + H2O
-
 Acinetobacter sp. CGMCC 0789 p-nitrophenol + laurate
-
 ?
p-nitrophenyl propionate + H2O
-
 Acinetobacter sp. p-nitrophenol + propionate
-
 ?
p-nitrophenyl propionate + H2O
-
 Acinetobacter sp. CGMCC 0789 p-nitrophenol + propionate
-
 ?

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Acinetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, 60% residual activity Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Acinetobacter sp.