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Literature summary for 3.1.1.59 extracted from

  • Tsubota, T.; Shimomura, M.; Ogura, T.; Seino, A.; Nakakura, T.; Mita, K.; Shinoda, T.; Shiotsuki, T.
    Molecular characterization and functional analysis of novel carboxyl/cholinesterases with GQSAG motif in the silkworm Bombyx mori (2010), Insect Biochem. Mol. Biol., 40, 100-112.
    View publication on PubMed
Search for more literature for 3.1.1.59

Cloned(Commentary)

Cloned (Comment) Organism
gene Bmcce-5, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis, recombinant expression of BmCCE-5 as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus system Bombyx mori
gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus system Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 juvenile hormone III pH 7.4, 22°C, recombinant BmCCE-5 Bombyx mori
0.0028
-
 juvenile hormone III pH 7.4, 22°C, recombinant BmJHE Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
juvenile hormone III + H2O Bombyx mori BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
 gene Bmjhe
-
Bombyx mori D2KWC2 gene Bmcce-5
-

Source Tissue

Source Tissue Comment Organism Textmining
gut strong expression of Bmcce-5 Bombyx mori
-
malpighian tubule strong expression of Bmcce-5 Bombyx mori
-
additional information developmental expression patterns of gene Bmcce-5 by real-time quantitative PCR analysis, the expression pattern varies among developmental stages and organs Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
juvenile hormone III + H2O
-
 Bombyx mori ?
-
 ?
juvenile hormone III + H2O BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm Bombyx mori ?
-
 ?
juvenile hormone III + H2O BmCCE-5 has the ability to degrade juvenile hormone, however, the protein might not function as a JH-specific esterase in vivo as it has a high Km value for juvenile hormone Bombyx mori ?
-
 ?
methyl heptylthioacetothioate + H2O a synthetic substrate Bombyx mori ?
-
 ?
additional information the enzymes encoded by genes Bmcce-1-4 are not active on juvenile hormone Bombyx mori ?
-
 ?

Synonyms

Synonyms Comment Organism
BmCCE-5
-
 Bombyx mori
JH-specific esterase
-
 Bombyx mori
JHE
-
 Bombyx mori
juvenile hormone-selective esterase
-
 Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Bombyx mori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013 0.018 juvenile hormone III pH 7.4, 22°C, recombinant BmCCE-5 Bombyx mori
1
-
 juvenile hormone III pH 7.4, 22°C, recombinant BmJHE Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bombyx mori

General Information

General Information Comment Organism
physiological function Bmcce-5 is strongly expressed in Malpighian tubules and the gut, it might function in digestion or xenobiotic metabolism Bombyx mori
physiological function BmJHE, a juvenile hormone-selective esterase, JHE, is important for juvenile hormone titer regulation in the silkworm Bombyx mori Bombyx mori