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Literature summary for 3.1.1.57 extracted from

  • Maruyama, K.; Shibayama, T.; Ichikawa, A.; Sakou, Y.; Yamada, S.; Sugisaki, H.
    Cloning and characterization of the genes encoding enzymes for the protocatechuate meta-degradation pathway of Pseudomonas ochraceae NGJ1 (2004), Biosci. Biotechnol. Biochem., 68, 1434-1441.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas straminea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.094
-
2-Pyrone-4,6-dicarboxylate pH 8.5, 25°C Pseudomonas straminea
0.025
-
4-oxalomesaconate pH 6.5, 25°C Pseudomonas straminea

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Pseudomonas straminea
34345
-
1 * 34345, calculated from the deduced amino acid sequence, native mass by gel filtration Pseudomonas straminea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-pyrone-4,6-dicarboxylate + H2O Pseudomonas straminea involved in the meta-degradation pathway for protocatechuate 4-oxalomesaconate
-
r
2-pyrone-4,6-dicarboxylate + H2O Pseudomonas straminea NGJ1 involved in the meta-degradation pathway for protocatechuate 4-oxalomesaconate
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas straminea
-
-
-
Pseudomonas straminea NGJ1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pseudomonas straminea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
49.6
-
recombinant protein from Escherichia coli, 25°C Pseudomonas straminea
495
-
purified recombinant protein from Escherichia coli, 25°C Pseudomonas straminea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-pyrone-4,6-dicarboxylate + H2O
-
Pseudomonas straminea 4-oxalomesaconate
-
r
2-pyrone-4,6-dicarboxylate + H2O involved in the meta-degradation pathway for protocatechuate Pseudomonas straminea 4-oxalomesaconate
-
r
2-pyrone-4,6-dicarboxylate + H2O
-
Pseudomonas straminea NGJ1 4-oxalomesaconate
-
r
2-pyrone-4,6-dicarboxylate + H2O involved in the meta-degradation pathway for protocatechuate Pseudomonas straminea NGJ1 4-oxalomesaconate
-
r

Subunits

Subunits Comment Organism
monomer 1 * 34345, calculated from the deduced amino acid sequence, native mass by gel filtration Pseudomonas straminea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
reverse reaction Pseudomonas straminea
8.5
-
forward reaction Pseudomonas straminea