General Stability | Organism |
---|---|
aged enzyme preparation is fully reactivated by preincubation at 24°C for 10 min with an excess amount of dithiothreitol, cysteine or GSH | Pseudomonas straminea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co(NO3)2 | inhibits hydrolysis reaction by 59.9% | Pseudomonas straminea | |
Cu(NO3)2 | inhibits synthesis reaction | Pseudomonas straminea | |
HgCl2 | - |
Pseudomonas straminea | |
Mn(NO3)2 | inhibits hydrolysis reaction by 62.1% | Pseudomonas straminea | |
additional information | monoiodoacetate had essentially no effect, Mg(NO3)2, Ni(NO3)2, Ca(NO3)2, Cr(NO3)3 and Fe(NO3)3 shows essentially no effect, KCN and various metal chelating reagents including EDTA, tiron, 1,10-phenanthroline, 2,2'-bipyridine, diethyldithiocarbonate and 8-hydroxyquinoline shows no significant effect | Pseudomonas straminea | |
N-ethylmaleimide | - |
Pseudomonas straminea | |
p-chloromercuribenzoate | - |
Pseudomonas straminea | |
Zn(NO3)2 | inhibits hydrolysis reaction | Pseudomonas straminea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
4-oxalmesaconate | pH 6.5, 24°C | Pseudomonas straminea | |
0.087 | - |
2-Pyrone-4,6-dicarboxylate | pH 8.5, 24°C | Pseudomonas straminea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
gel filtration | Pseudomonas straminea |
33000 | - |
1 * 33000, SDS-PAGE | Pseudomonas straminea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-pyrone-4,6-dicarboxylate + H2O | Pseudomonas straminea | highly specific, various other lactones does not serve as substrates | 4-carboxy-2-hydroxyhexa-2,4-dienedioate | product isomerizes to 4-oxalmesaconate | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas straminea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas straminea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
247 | - |
purification of hydrolase activity | Pseudomonas straminea |
Storage Stability | Organism |
---|---|
-20°C, 30% of the activity is lost on storage for 1 year | Pseudomonas straminea |
-20°C, purified enzyme can be stored for at least 2 months without appreciable loss in activity | Pseudomonas straminea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-pyrone-4,6-dicarboxylate + H2O | highly specific | Pseudomonas straminea | 4-carboxy-2-hydroxyhexa-2,4-dienedioate | product isomerizes to 4-oxalmesaconate | r | |
2-pyrone-4,6-dicarboxylate + H2O | highly specific, various other lactones does not serve as substrates | Pseudomonas straminea | 4-carboxy-2-hydroxyhexa-2,4-dienedioate | product isomerizes to 4-oxalmesaconate | r | |
additional information | no hydrolysis of gamma-butyrolactone, gamma-valerolactone, epsilon-caprolactone, gamma-n-decalactone, D-glucorono-gamma-lactone, mevalonolactone, 2-pyrone-6-carboxylate, coumalic acid, isodehydracetic acid, 4-hydroxy-6-methyl-2-pyrone and 4-methoxy-6-methyl-2-H-pyran-2-one | Pseudomonas straminea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE | Pseudomonas straminea |
Synonyms | Comment | Organism |
---|---|---|
2-pyrone-4,6-dicarboxylate hydrolase | - |
Pseudomonas straminea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | 2-pyrone-4,6-dicarboxylate synthesis | Pseudomonas straminea |
8.5 | - |
2-pyrone-4,6-dicarboxylate hydrolysis | Pseudomonas straminea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas straminea | isolelectric focusing at 6°C, LKB 8101 column pH 3.5-10.0 | - |
5.49 |