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Literature summary for 3.1.1.56 extracted from
- Purification and properties of an esterase from the yeast Saccharomyces cerevisiae and identification of the encoding gene (1999), Appl. Environ. Microbiol., 65, 3470-3472.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.056 | - |
carboxyfluorescin diacetate | pH 7.0, 37°C | Saccharomyces cerevisiae |  |
| 0.076 | - |
4-yethylumbelliferyl acetate | pH 7.0, 37°C | Saccharomyces cerevisiae | |
| 0.35 | - |
p-nitrophenyl acetate | pH 7.0, 37°C | Saccharomyces cerevisiae | |
| 0.86 | - |
1-naphthyl acetate | pH 7.0, 37°C | Saccharomyces cerevisiae | |
| 0.88 | - |
S-formylglutathione | pH 7.0, 30°C | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl acetate + H2O | Saccharomyces cerevisiae | - |
4-methylumbelliferone + acetate | - |
? | |
| additional information | Saccharomyces cerevisiae | the enzyme catalyses the hydrolysis of S-formylglutathione, a role in formaldehyde detoxification is possible | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Saccharomyces cerevisiae | 1-naphthol + acetate | - |
? | |
| 4-methylumbelliferyl acetate + H2O | - |
Saccharomyces cerevisiae | 4-methylumbelliferone + acetate | - |
? | |
| carboxyfluorescein diacetate + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| carboxyfluorescin diacetate + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | the enzyme catalyses the hydrolysis of S-formylglutathione, a role in formaldehyde detoxification is possible | Saccharomyces cerevisiae | ? | - |
? | |
| p-nitrophenyl acetate + H2O | - |
Saccharomyces cerevisiae | p-nitrophenol + acetate | - |
? | |
| S-formylglutathione + H2O | - |
Saccharomyces cerevisiae | formate + glutathione | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Saccharomyces cerevisiae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharomyces cerevisiae | isoelectric focusing | - |
5 |
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