Any feedback?
Literature summary for 3.1.1.53 extracted from
- Sialate 9-O-acetylesterase from rat liver (1989), Methods Enzymol., 179, 409-415.
General Stability
| General Stability | Organism |
|---|---|
| activity becomes unstable on excessive dilution | Rattus norvegicus |
| lyophilization and reconstitution results in loss of 30% of activity | Rattus norvegicus |
| single cycle of freeze-thaw results in little loss of activity, repeated freeze-thaw results in gradual loss of activity | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 9-acetamido-N-acetyl-O-acetylneuraminate | little effect at 5 mM | Rattus norvegicus |  |
| bis-p-nitrophenyl phosphate | little effect on the activity | Rattus norvegicus | |
| CHAPS | lowers the activity by 40% | Rattus norvegicus | |
| deoxycholate | 75-80% loss of activity at 2% concentration | Rattus norvegicus | |
| Diethyl-4-nitrophenylphosphate | irreversibly inactivated | Rattus norvegicus | |
| diisopropyl fluorophosphate | irreversibly inactivated | Rattus norvegicus | |
| Hg2+ | little effect on the activity | Rattus norvegicus | |
| additional information | not affected by detergents, i.e. Triton X-100, Triton CF-54, saponin | Rattus norvegicus | |
| p-chloromercuribenzoate | little effect on the activity | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi vesicle | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
1 * 30000 + 1 * 36000 | Rattus norvegicus |
| 36000 | - |
1 * 30000 + 1 * 36000 | Rattus norvegicus |
| 61000 | - |
- |
Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | acetone powder of the glycosylated form of the enzyme | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.84 | - |
4-methylumbelliferyl acetate as substrate | Rattus norvegicus |
| 11.6 | - |
alpha-naphthyl acetate as substrate | Rattus norvegicus |
| 12.8 | - |
4-nitrophenyl acetate as substrate | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, concentrated purified enzyme, stable for at least 1 month | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Rattus norvegicus | 4-nitrophenol + acetate | - |
? | |
| alpha-naphthyl acetate + H2O | - |
Rattus norvegicus | 1-naphthol + acetate | - |
? | |
| N-acetyl-9-O-acetylneuraminate + H2O | - |
Rattus norvegicus | N-acetylneuraminate + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 30000 + 1 * 36000 | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sialate 9-O-acetylesterase | - |
Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
40% activity is lost in 20 h | Rattus norvegicus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
