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Literature summary for 3.1.1.53 extracted from

  • Schauer, R.; Reuter, G.; Stoll, S.
    Sialate O-acetylesterases: key enzymes in sialic acid catabolism (1988), Biochimie, 70, 1511-1519.
    View publication on PubMed
Search for more literature for 3.1.1.53

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Equus caballus
Cu2+
-
 influenza C virus
Diethyl-4-nitrophenylphosphate 50% inhibition at 0.0024 mM Bos taurus
Diethyl-4-nitrophenylphosphate
-
 Equus caballus
Diethyl-4-nitrophenylphosphate 50% inhibition at 0.24 mM influenza C virus
diisopropyl fluorophosphate 50% inhibition at 0.35 mM Bos taurus
diisopropyl fluorophosphate
-
 Equus caballus
diisopropyl fluorophosphate 50% inhibition at 0.01 mM influenza C virus
Hg2+ 50% inhibition at 0.0035 mM Bos taurus
Hg2+
-
 Equus caballus
Hg2+
-
 influenza C virus
N-acetyl-9-O-acetylneuraminate substrate inhibition above 2 mM influenza C virus
physostigmine 25% inhibition at 10 mM Bos taurus
physostigmine 25% inhibition at 10 mM influenza C virus
Zn2+
-
 Equus caballus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 4-yethylumbelliferyl acetate
-
 influenza C virus
0.17
-
 4-yethylumbelliferyl acetate
-
 Bos taurus
0.8
-
 N-acetyl-9-O-acetylneuraminic acid
-
 influenza C virus
0.9
-
 N-acetyl-9-O-lactoylneuraminic acid
-
 influenza C virus
1.1
-
 N-acetyl-9-O-acetylneuraminic acid
-
 Equus caballus
18
-
 N-acetyl-9-O-acetylneuraminic acid
-
 Bos taurus
24
-
 N-acetyl-9-O-lactoylneuraminic acid
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 1 * 30000 + 1 * 65000, SDS-PAGE influenza C virus
56500
-
 gel filtration Equus caballus
56500
-
 gel filtration and SDS-PAGE Bos taurus
65000
-
 1 * 30000 + 1 * 65000, SDS-PAGE influenza C virus
88000
-
 gel fitration influenza C virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus O-acetylated sialic acids ?
-
 ?
additional information Equus caballus O-acetylated sialic acids ?
-
 ?
additional information influenza C virus O-acetylated sialic acids ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Equus caballus
-
-
-
influenza C virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
180fold enrichment influenza C virus
2600fold enrichment Bos taurus
850fold enrichment Equus caballus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Bos taurus
-
liver
-
 Equus caballus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-2,3-didehydro-N-acetyl-9-O-acetylneuraminate + H2O 55% of the activity compared to N-acetyl-9-O-acetylneuraminate Bos taurus ?
-
 ?
2-deoxy-2,3-didehydro-N-acetyl-9-O-acetylneuraminate + H2O 37% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus ?
-
 ?
4-methylumbelliferyl acetate + H2O 130% of the activity compared to N-acetyl-9-O-acetylneuraminate Bos taurus 4-methylumbelliferone + acetate
-
 ?
4-methylumbelliferyl acetate + H2O 220% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus 4-methylumbelliferone + acetate
-
 ?
4-methylumbelliferyl butyrate + H2O 14% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus 4-methylumbelliferone + butyrate
-
 ?
4-nitrophenyl acetate + H2O 3500% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus 4-nitrophenol + acetate
-
 ?
4-nitrophenyl acetate + H2O 680% of the activity compared to N-acetyl-9-O-acetylneuraminate Bos taurus 4-nitrophenol + acetate
-
 ?
acetyl-CoA + H2O 2% of the activity compared to N-acetyl-9-O-acetylneuraminate Bos taurus CoA + acetate
-
 ?
alpha-naphthyl acetate + H2O 2200% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus 1-naphthol + acetate
-
 ?
alpha-naphthyl acetate + H2O 643% of the activity compared to N-acetyl-9-O-acetylneuraminate Bos taurus 1-naphthol + acetate
-
 ?
bovine submandibular gland mucin + H2O 30% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus ?
-
 ?
additional information no hydrolysis with N-acetyl-4-O-lactoylneuraminate, N-acetyl-9-O-acetylneuraminate methyl ester Bos taurus ?
-
 ?
additional information no hydrolysis with N-acetyl-4-O-lactoylneuraminate, N-acetyl-9-O-acetylneuraminate methyl ester influenza C virus ?
-
 ?
additional information no hydrolysis with acetyl-coenzyme A, acetylthiocholine iodide influenza C virus ?
-
 ?
additional information no hydrolysis with N-glycoloyl-9-O-lactoylneuraminate, bovine submandibular gland mucin, rat serum glycoprotein, rat erythrocytes Bos taurus ?
-
 ?
additional information no hydrolysis with N-acetyl-7-O-acetylneuraminate, horse alpha1-acid glycoprotein, N-acetylneuraminate methyl ester Bos taurus ?
-
 ?
additional information no hydrolysis with N-acetyl-7-O-acetylneuraminate, horse alpha1-acid glycoprotein, N-acetylneuraminate methyl ester Equus caballus ?
-
 ?
additional information no hydrolysis with N-acetyl-7-O-acetylneuraminate, horse alpha1-acid glycoprotein, N-acetylneuraminate methyl ester influenza C virus ?
-
 ?
additional information O-acetylated sialic acids Bos taurus ?
-
 ?
additional information O-acetylated sialic acids Equus caballus ?
-
 ?
additional information O-acetylated sialic acids influenza C virus ?
-
 ?
N-acetyl-4-O-acetylneuraminate + H2O 60% of the activity compared to N-acetyl-9-O-acetylneuraminate Bos taurus N-acetylneuraminate + acetate
-
 ?
N-acetyl-4-O-acetylneuraminate + H2O 3% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus N-acetylneuraminate + acetate
-
 ?
N-acetyl-4-O-acetylneuraminate + H2O no hydrolysis at pI 4.8, same rate of hydrolysis at pI 5.7 compared to N-acetyl-9-O-acetylneuraminate Equus caballus N-acetylneuraminate + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate + H2O
-
 Bos taurus N-acetylneuraminate + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate + H2O
-
 influenza C virus N-acetylneuraminate + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate + H2O at pI 4.8 and pI 5.7 Equus caballus N-acetylneuraminate + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate beta-methylglycoside + H2O 70% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus N-acetylneuraminate beta-methylglycoside + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate lactose + H2O 96% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus N-acetylneuraminate lactose + acetate
-
 ?
N-acetyl-9-O-acetylneuraminate lactose + H2O same rate of hydrolysis compared to N-acetyl-9-O-acetylneuraminate Bos taurus N-acetylneuraminate lactose + acetate
-
 ?
N-acetyl-9-O-lactoylneuraminic acid + H2O
-
 Bos taurus 9-O-lactoylneuraminic acid + acetate
-
 ?
N-acetyl-9-O-lactoylneuraminic acid + H2O
-
 influenza C virus 9-O-lactoylneuraminic acid + acetate
-
 ?
rat serum glycoprotein + H2O 90% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus ?
-
 ?
thiophenyl acetate + H2O 12% of the activity compared to N-acetyl-9-O-acetylneuraminate influenza C virus thiophenol + acetate
-
 ?
thiophenyl acetate + H2O 305% of the activity compared to N-acetyl-9-O-acetylneuraminate Bos taurus thiophenol + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 30000 + 1 * 65000, SDS-PAGE influenza C virus
monomer 1* 56500, gel filtration and SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
 Bos taurus
7.8
-
-
 influenza C virus
8.5
-
-
 Equus caballus