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Literature summary for 3.1.1.53 extracted from

  • Phansopa, C.; Kozak, R.P.; Liew, L.P.; Frey, A.M.; Farmilo, T.; Parker, J.L.; Kelly, D.J.; Emery, R.J.; Thomson, R.I.; Royle, L.; Gardner, R.A.; Spencer, D.I.; Stafford, G.P.
    Characterization of a sialate-O-acetylesterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid release by NanH, its cognate sialidase (2015), Biochem. J., 472, 157-67 .
    View publication on PubMed
Search for more literature for 3.1.1.53

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Tannerella forsythia

General Stability

General Stability Organism
23, 72 h, very little loss of activity Tannerella forsythia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information presence of two potential catalytic domains, but no evidence is found that any cooperativity between these potential domains exists. A Hill number of 0.97 is found for substrate 4-nitrophenyl acetate, pH 7.4, 25°C Tannerella forsythia
0.0512
-
 4-nitrophenyl acetate pH 7.4, 25°C Tannerella forsythia

Organism

Organism UniProt Comment Textmining
Tannerella forsythia A0A1D3US71
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a strong putative N-terminal secretion signal with a potential cleavage site after alanine 18 Tannerella forsythia

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Tannerella forsythia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O
-
 Tannerella forsythia 4-nitrophenol + acetate
-
 ?
5-N-acetyl-9-O-acetylneuraminic acid + H2O
-
 Tannerella forsythia 5-N-acetylneuraminic acid + acetate
-
 ?
5-N-glycolyl-9-O-acetylneuraminic acid + H2O
-
 Tannerella forsythia 5-N-glycolylneuraminic acid + acetate
-
 ?
additional information NanS can act upon complex N-glycans released from erythropoietin, bovine submaxillary mucin and oral epithelial cell-bound glycans. When incubated with its cognate sialidase, NanS increases sialic acid release from mucin and oral epithelial cell surfaces. Enzyme does not remove acetyl groups at the 4-O position Tannerella forsythia ?
-
 ?

Subunits

Subunits Comment Organism
? x * 76300, calculated from sequence, putative mature protein Tannerella forsythia

Synonyms

Synonyms Comment Organism
NANS
-
 Tannerella forsythia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.2 7.6
-
 Tannerella forsythia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6 8.4
-
 Tannerella forsythia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
111
-
 4-nitrophenyl acetate pH 7.4, 25°C Tannerella forsythia

General Information

General Information Comment Organism
physiological function when incubated with its cognate sialidase, NanS increases sialic acid release from mucin and oral epithelial cell surfaces. The esterase improves sialic acid harvesting for Tannerella forsythia and potentially other members of the oral microbiome Tannerella forsythia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2200
-
 4-nitrophenyl acetate pH 7.4, 25°C Tannerella forsythia