Cloned (Comment) | Organism |
---|---|
in pBACgus-6, -3, -1 for baculovirus-mediated expression for 4 h at 20°C with C-terminal hexa-His-tag in insect Sf9 cells, deletion of transmembrane region and cytoplasmic tail | Isavirus salaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 150 mM, inhibitory at 500 mM when pH below pH 8 | Isavirus salaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37230 | - |
MALDI-TOF mass spectrometry | Isavirus salaris |
37700 | - |
theoretical | Isavirus salaris |
40000 | - |
SDS-PAGE, purified, recombinant protein | Isavirus salaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Isavirus salaris | - |
ISAV 4, isolate 4, Glesvaer/2/90 | - |
Oxidation Stability | Organism |
---|---|
contains disulfide bonds | Isavirus salaris |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | purified, recombinant protein, deglycosylation with N-glycosidase F | Isavirus salaris |
Purification (Comment) | Organism |
---|---|
nickel-affinity chromatography, elution with 500 mM imidazole, pH 7.5, yield of 300 microg soluble recombinant protein per litre Sf9 cell culture (3 mg/l insoluble) | Isavirus salaris |
Storage Stability | Organism |
---|---|
-20°C, 20 mM HEPES 150 mM NaCl pH 8, 3 weeks, fully active | Isavirus salaris |
4°C, 20 mM HEPES 150 mM NaCl pH 8, 1 week, fully active | Isavirus salaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl acetate + H2O | pH 8 | Isavirus salaris | p-nitrophenol + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HE | - |
Isavirus salaris |
hemagglutinin esterase | - |
Isavirus salaris |
recHE4 | recombinant HE | Isavirus salaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
HEPES buffer, activity affected at high NaCl concentrations (500 mM) | Isavirus salaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.2 | HEPES buffer | Isavirus salaris |