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Literature summary for 3.1.1.53 extracted from

  • Usui, S.; Kubota, M.; Iguchi, K.; Kiho, T.; Sugiyama, T.; Katagiri, Y.; Hirano, K.
    Sialic acid 9-O-acetylesterase catalyzes the hydrolyzing reaction from alacepril to deacetylalacepril (2003), Pharm. Res., 20, 1309-1316.
    View publication on PubMed
Search for more literature for 3.1.1.53

Inhibitors

Inhibitors Comment Organism Structure
Alpha-naphthyl acetate competitive inhibitor Rattus norvegicus
Cu2+ complete inhibition at 1 mM Rattus norvegicus
diisopropyl fluorophosphates complete inhibition at 1 mM Rattus norvegicus
Hg2+ complete inhibition at 1 mM Rattus norvegicus
phenylmethylsulfonyl fluoride complete inhibition at 10 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.652
-
 spironolactone pH 7.5, 37°C Rattus norvegicus
1.28
-
 acetyl-CoA pH 7.5, 37°C Rattus norvegicus
1.71
-
 Alpha-naphthyl acetate pH 7.5, 37°C Rattus norvegicus
22
-
 alacepril pH 7.5, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
microsome only little activity Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 1 * 29000 + 1 * 36000, SDS-PAGE Rattus norvegicus
36000
-
 1 * 29000 + 1 * 36000, SDS-PAGE Rattus norvegicus
60000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male Sprague-Dawley
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.695
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O
-
 Rattus norvegicus CoA + acetate
-
 ?
alacepril + H2O
-
 Rattus norvegicus deacetylalacepril + acetate
-
 ?
alpha-naphthyl acetate + H2O
-
 Rattus norvegicus alpha-naphthol + acetate
-
 ?
spironolactone + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 29000 + 1 * 36000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 room temperature, complete inactivation in the presence of 10 mM 2-mercaptoethanol after 8 h Rattus norvegicus