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Literature summary for 3.1.1.50 extracted from

  • Kalinowska, M.; Wojciechowski, Z.A.
    Characterization of wax-ester hydrolase from roots of white mustard (Sinapis alba L.) seedlings (1985), Acta Biochim. Pol., 32, 263-269.
No PubMed abstract available
Search for more literature for 3.1.1.50

Activating Compound

Activating Compound Comment Organism Structure
deoxycholate
-
 Sinapis alba
DTT 5 mM, 40% stimulation Sinapis alba
Triton X-100 stimulates Sinapis alba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 cholesteryl palmitate
-
 Sinapis alba
0.028
-
 hexadecanyl palmitate
-
 Sinapis alba

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Sinapis alba 16020
-

Organism

Organism UniProt Comment Textmining
Sinapis alba
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sinapis alba

Source Tissue

Source Tissue Comment Organism Textmining
root seedlings Sinapis alba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.074
-
-
 Sinapis alba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyl palmitate + H2O 76% of the activity with cholesteryl palmitate Sinapis alba butanol + palmitate
-
 ?
cholesteryl palmitate + H2O
-
 Sinapis alba cholesterol + palmitate
-
 ?
decanyl palmitate + H2O 84% of the activity with cholesteryl palmitate Sinapis alba decanol + palmitate
-
 ?
docosanyl palmitate + H2O 69% of the activity with cholesteryl palmitate Sinapis alba docosanol + palmitate
-
 ?
dodecanyl palmitate + H2O 85% of the activity with cholesteryl palmitate Sinapis alba dodecanol + palmitate
-
 ?
eicosanyl palmitate + H2O 72% of the activity with cholesteryl palmitate Sinapis alba eicosanol + palmitate
-
 ?
ethyl palmitate + H2O 69% of the activity with cholesteryl palmitate Sinapis alba ethanol + palmitate
-
 ?
hexadecanyl acetate + H2O 3% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + acetate
-
 ?
hexadecanyl arachidate + H2O 27% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + arachidate
-
 ?
hexadecanyl behenic acid ester + H2O 21% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + behenic acid
-
 ?
hexadecanyl butyrate + H2O 10% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + butyrate
-
 ?
hexadecanyl caprylate + H2O 25% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + caprylate
-
 ?
hexadecanyl laurate + H2O 32% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + laurate
-
 ?
hexadecanyl myristate + H2O 36% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + myristate
-
 ?
hexadecanyl palmitate + H2O
-
 Sinapis alba hexadecanol + palmitate
-
 ?
hexadecanyl palmitate + H2O 77% of the activity with cholesteryl palmitate Sinapis alba hexadecanol + palmitate
-
 ?
hexadecanyl stearate + H2O 48% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + stearate
-
 ?
octadecanyl palmitate + H2O 72% of the activity with cholesteryl palmitate Sinapis alba octadecanol + palmitate
-
 ?
octanyl palmitate + H2O 92% of the activity with cholesteryl palmitate Sinapis alba octanol + palmitate
-
 ?
tetraeicosanyl palmitate + H2O 61% of the activity with cholesteryl palmitate Sinapis alba tetraeicosanol + palmitate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 8.5 broad optimum without definite maximum peak Sinapis alba

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 activity sharply decreases below pH 5.5. and above pH 9.0 Sinapis alba