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Literature summary for 3.1.1.5 extracted from

  • Hirano, T.; Kishi, M.; Sugimoto, H.; Taguchi, R.; Obinata, H.; Ohshima, N.; Tatei, K.; Izumi, T.
    Thioesterase activity and subcellular localization of acylprotein thioesterase 1/lysophospholipase 1 (2009), Biochim. Biophys. Acta, 1791, 797-805.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
GST-fusion protein expressed in Escherichia coli BL21, expressed in HEK-293 cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
S119A part of the catalytic triad, no activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-palmitoyl lysophosphatidylcholine competitive inhibitor for acylprotein thioesterase activity Homo sapiens
palmitoyl-peptide competitive inhibitor for lysophospholipase activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00349
-
palmitoyl-peptide
-
Homo sapiens
0.0273
-
1-palmitoyl lysophosphatidylcholine
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity independent of Ca2+ and Mg2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
x * 25000, SDS-PAGE (52000 for GST-fusion protein) Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6IAQ1
-
-

Purification (Commentary)

Purification (Comment) Organism
GST affinity chromatography, GST-tag cleaved from GST-fusion protein Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.62
-
substrate 1-palmitoyl lysophosphatidylcholine Homo sapiens
27.3
-
substrate palmitoyl-peptide Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, 50 mM Tris/HCl, pH 7.4, more than one year, stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-palmitoyl lysophosphatidylcholine + H2O
-
Homo sapiens glycerophosphocholine + palmitate
-
?
palmitoyl-peptide + H2O residue 3 to 14 of RGS4 protein with palmitoyl group attached at Cys12 of RGS4 protein Homo sapiens palmitate + peptide
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE (52000 for GST-fusion protein) Homo sapiens

Synonyms

Synonyms Comment Organism
acylprotein thioesterase 1
-
Homo sapiens
lysophospholipase 1
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0222
-
1-palmitoyl lysophosphatidylcholine for acylprotein thioesterase activity Homo sapiens
0.0323
-
palmitoyl-peptide for lysophospholipase activity Homo sapiens