Cloned (Comment) | Organism |
---|---|
GST-fusion protein expressed in Escherichia coli BL21, expressed in HEK-293 cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S119A | part of the catalytic triad, no activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-palmitoyl lysophosphatidylcholine | competitive inhibitor for acylprotein thioesterase activity | Homo sapiens | |
palmitoyl-peptide | competitive inhibitor for lysophospholipase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00349 | - |
palmitoyl-peptide | - |
Homo sapiens | |
0.0273 | - |
1-palmitoyl lysophosphatidylcholine | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity independent of Ca2+ and Mg2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, SDS-PAGE (52000 for GST-fusion protein) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6IAQ1 | - |
- |
Purification (Comment) | Organism |
---|---|
GST affinity chromatography, GST-tag cleaved from GST-fusion protein | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.62 | - |
substrate 1-palmitoyl lysophosphatidylcholine | Homo sapiens |
27.3 | - |
substrate palmitoyl-peptide | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, 50 mM Tris/HCl, pH 7.4, more than one year, stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-palmitoyl lysophosphatidylcholine + H2O | - |
Homo sapiens | glycerophosphocholine + palmitate | - |
? | |
palmitoyl-peptide + H2O | residue 3 to 14 of RGS4 protein with palmitoyl group attached at Cys12 of RGS4 protein | Homo sapiens | palmitate + peptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25000, SDS-PAGE (52000 for GST-fusion protein) | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
acylprotein thioesterase 1 | - |
Homo sapiens |
lysophospholipase 1 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0222 | - |
1-palmitoyl lysophosphatidylcholine | for acylprotein thioesterase activity | Homo sapiens | |
0.0323 | - |
palmitoyl-peptide | for lysophospholipase activity | Homo sapiens |