Cloned (Comment) | Organism |
---|---|
into the vector pGEX-KG and subsequently into pET23a for expression in Escherichia coli BL21DE3 pLysS cells | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylmethylsulfonyl fluoride | PMSF | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
determined by SDS-PAGE | Pyrococcus furiosus |
32040 | - |
calculated | Pyrococcus furiosus |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 25%, substrate p-nitrophenyl palmitate, relative activiy 58% | Pyrococcus furiosus |
Ethanol | 25%, substrate p-nitrophenyl palmitate, relative activiy 39% | Pyrococcus furiosus |
Methanol | 25%, substrate p-nitrophenyl palmitate, relative activiy 20% | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8TZI9 | - |
- |
Purification (Comment) | Organism |
---|---|
using a Ni-NTA affiniity column | Pyrococcus furiosus |
Renatured (Comment) | Organism |
---|---|
the enzyme is refolded from inclusion bodies | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl acetate + H2O | substrate activity assay | Pyrococcus furiosus | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl butyrate | substrate activity assay | Pyrococcus furiosus | p-nitrophenol + butyrate | - |
? | |
p-nitrophenyl palmitate | substrate activity assay | Pyrococcus furiosus | p-nitrophenol + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | prediction, based on the determination of the hydrodynamic radius by dynamic light scattering measurements | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
lysophospholipase | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | conformational changes are negligible over the temperature range of 30-80°C | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
begins to unfold above 85°C, with unfolding remaining incomplete even at 95°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pyrococcus furiosus |