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Literature summary for 3.1.1.5 extracted from

  • Chandrayan, S.K.; Dhaunta, N.; Guptasarma, P.
    Expression, purification, refolding and characterization of a putative lysophospholipase from Pyrococcus furiosus: retention of structure and lipase/esterase activity in the presence of water-miscible organic solvents at high temperatures (2008), Protein Expr. Purif., 59, 327-333.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEX-KG and subsequently into pET23a for expression in Escherichia coli BL21DE3 pLysS cells Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
phenylmethylsulfonyl fluoride PMSF Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
determined by SDS-PAGE Pyrococcus furiosus
32040
-
calculated Pyrococcus furiosus

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 25%, substrate p-nitrophenyl palmitate, relative activiy 58% Pyrococcus furiosus
Ethanol 25%, substrate p-nitrophenyl palmitate, relative activiy 39% Pyrococcus furiosus
Methanol 25%, substrate p-nitrophenyl palmitate, relative activiy 20% Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8TZI9
-
-

Purification (Commentary)

Purification (Comment) Organism
using a Ni-NTA affiniity column Pyrococcus furiosus

Renatured (Commentary)

Renatured (Comment) Organism
the enzyme is refolded from inclusion bodies Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl acetate + H2O substrate activity assay Pyrococcus furiosus p-nitrophenol + acetate
-
?
p-nitrophenyl butyrate substrate activity assay Pyrococcus furiosus p-nitrophenol + butyrate
-
?
p-nitrophenyl palmitate substrate activity assay Pyrococcus furiosus p-nitrophenol + palmitate
-
?

Subunits

Subunits Comment Organism
homodimer prediction, based on the determination of the hydrodynamic radius by dynamic light scattering measurements Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
lysophospholipase
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 conformational changes are negligible over the temperature range of 30-80°C Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
begins to unfold above 85°C, with unfolding remaining incomplete even at 95°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Pyrococcus furiosus