Any feedback?
Literature summary for 3.1.1.5 extracted from
- Lysophospholipase from the human blood fluke, Schistosoma japonicum (2007), Int. J. Infect. Dis., 12, 143-151.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | Schistosome lysophospholipase may represent a new target for anti-schistosomal chemotherapy | Schistosoma japonicum |
| medicine | schistosome lysophospholipase may represent a new target for anti-schistosomal-chemotherapy | Schistosoma japonicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Schistosoma japonicum |
| expressed in Escherichia coli as a His-tagged fusion protein | Schistosoma japonicum |
| into the vector pQE30 for expression in Escherichia coli cells | Schistosoma japonicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
determined by Western blot analysis, extract of adult Schistosoma japonicum worm | Schistosoma japonicum |
| 23000 | - |
immunoblot analysis, single band in both membrane-associated and soluble tissue fractions of adult schistosomes | Schistosoma japonicum |
| 24840 | - |
calculated | Schistosoma japonicum |
| 24840 | - |
calculated from cDNA | Schistosoma japonicum |
| 26000 | - |
SDS-PAGE, recombinant protein | Schistosoma japonicum |
| 26000 | - |
determined by SDS-PAGE, recombinant 6-His-tagged lysophospholipase | Schistosoma japonicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-acyl-2-lysophosphatidylcholine + H2O | Schistosoma japonicum | - |
fatty acid + glycerophosphocholine | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schistosoma japonicum | - |
- |
- |
| Schistosoma japonicum | Q9U8F2 | - |
- |
| Schistosoma japonicum | Q9U8F2 | enzyme exhibits the GXSXG consensus active site characteristic of serine proteases, esterases, and lipases, and includes the catalytic triad motif of SerAspHis residues characteristic of serine hydrolases. Three-dimensional structural predictions indicate that the putative catalytic triad formed by these three residues is located at the alpha/beta-hydrolase fold characteristic of the lipases and esterases | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by affinity chromatography on Ni-NTA Superflow resin | Schistosoma japonicum |
| using Ni-NTA chromatography | Schistosoma japonicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adult | - |
Schistosoma japonicum | - |
| egg | - |
Schistosoma japonicum | - |
| egg | transcript is isolated from the egg stage | Schistosoma japonicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-acyl-2-lysophosphatidylcholine + H2O | - |
Schistosoma japonicum | fatty acid + glycerophosphocholine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lysophospholipase | - |
Schistosoma japonicum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Schistosoma japonicum | determined by isoelectric focusing, SDS-PAGE, extract of adult Schistosoma japonicum worm | - |
6.05 |
| Schistosoma japonicum | predicted | - |
7.06 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
