Literature summary for 3.1.1.5 extracted from

Tamura, M.; Ajayi, T.; Allmond, L.R.; Moriyama, K.; Wiener-Kronish, J.P.; Sawa, T.
Lysophospholipase A activity of Pseudomonas aeruginosa type III secretory toxin ExoU (2004), Biochem. Biophys. Res. Commun., 316, 323-331.

Search for more literature for 3.1.1.5

Activating Compound

Activating Compound	Comment	Organism	Structure
glycerol	50% increase of lysophospholipase activity in the presence of 30% glycerol	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
arachidonyl trifluoromethyl ketone	0.025 mM, approx. 50% inhibition	Pseudomonas aeruginosa
bromoenol lactone	0.025 mM, approx. 30% inhibition	Pseudomonas aeruginosa
trifluoromethyl ketone	0.025 mM, approx. 40% inhibition	Pseudomonas aeruginosa
Triton X-100	-	Pseudomonas aeruginosa

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	Ca2+ independent phospholipase A2 activity	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-lysophosphatidylcholine + H2O	Pseudomonas aeruginosa	-	glycerophosphocholine + a carboxylate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant ExoU	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-palmitylphosphatidylcholine + H2O	low activity after activating of ExoU with an unknown eukaryotic cell factor	Pseudomonas aeruginosa	1-palmityl-lysophosphatidylcholine + palmitic acid	-	?
1-palmitoyl-lysophosphatidylcholine + H2O	-	Pseudomonas aeruginosa	glycerophosphocholine + palmitic acid	-	?
2-lysophosphatidylcholine + H2O	-	Pseudomonas aeruginosa	glycerophosphocholine + a carboxylate	-	?

Synonyms

Synonyms	Comment	Organism
ExoU	-	Pseudomonas aeruginosa
lysoPLA	-	Pseudomonas aeruginosa

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)