Cloned (Comment) | Organism |
---|---|
functional expression of Brassica napus BnSCE3 in Arabidopsis thaliana developing seeds resulting in a dramatic decrease in the sinapine content | Brassica napus |
gene At1g28640, the BnSCE3 homologue is tagged on chromosome I | Arabidopsis thaliana |
gene At1g28650, the BnSCE3 homologue is tagged on chromosome I | Arabidopsis thaliana |
gene At1g28660, the BnSCE3 homologue is tagged on chromosome I | Arabidopsis thaliana |
gene At1g28670, the BnSCE3 homologue is tagged on chromosome I | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Brassica napus | BnSCE3 reveals a broad substrate specificity toward phenolic choline esters, even accepting phosphatidylcholine, pointing to a phospholipase A-like activity | ? | - |
? | |
O-sinapoylcholine + H2O | Brassica napus | i.e. sinapine | choline + sinapate | - |
? | |
O-sinapoylcholine + H2O | Arabidopsis thaliana | i.e. sinapine | choline + sinapate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P0C8Z7 | gene At1g28640 | - |
Arabidopsis thaliana | Q38894 | gene At1g28670 | - |
Arabidopsis thaliana | Q3E7I6 | gene At1g28650 | - |
Arabidopsis thaliana | Q9FPE4 | At1g28660 | - |
Brassica napus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | germinating | Brassica napus | - |
seed | germinating | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | BnSCE3 reveals a broad substrate specificity toward phenolic choline esters, even accepting phosphatidylcholine, pointing to a phospholipase A-like activity | Brassica napus | ? | - |
? | |
O-sinapoylcholine + H2O | i.e. sinapine | Brassica napus | choline + sinapate | - |
? | |
O-sinapoylcholine + H2O | i.e. sinapine | Arabidopsis thaliana | choline + sinapate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCE | - |
Brassica napus |
SCE | - |
Arabidopsis thaliana |
sinapine esterase | - |
Brassica napus |
sinapoylcholine(sinapine) esterase | - |
Brassica napus |
sinapoylcholine(sinapine) esterase | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | BnSCE3 is classifed as a member of the SGNH hydrolase subfamily of GDSL-lipases which cleave ester bonds in a wide array of lipids. Sinapine esterase BnSCE3 kept the catalytic mechanism of the hydrolytic ancestor but experienced a change in substrate specificity | Brassica napus |
evolution | sinapine esterase is classifed as a member of the SGNH hydrolase subfamily of GDSL-lipases which cleave ester bonds in a wide array of lipids | Arabidopsis thaliana |
metabolism | 1-O-sinapoylglucose, produced by UDP-glucose:sinapate glucosyltransferase during seed development, is converted to sinapine by sinapoylglucose:choline sinapoyltransferase and hydrolyzed by sinapoylcholine (sinapine) esterase in germinating seeds. The released sinapate feeds via sinapoylglucose into the biosynthesis of sinapoylmalate in the seedlings catalyzed by sinapoylglucose:L-malate sinapoyltransferase. Pathway of sinapate ester biosynthesis, overview | Brassica napus |
metabolism | 1-O-sinapoylglucose, produced by UDP-glucose:sinapate glucosyltransferase during seed development, is converted to sinapine by sinapoylglucose:choline sinapoyltransferase and hydrolyzed by sinapoylcholine (sinapine) esterase in germinating seeds. The released sinapate feeds via sinapoylglucose into the biosynthesis of sinapoylmalate in the seedlings catalyzed by sinapoylglucose:L-malate sinapoyltransferase. Pathway of sinapate ester biosynthesis, overview | Arabidopsis thaliana |
additional information | SCE activity in seedlings of Brassica napus is mediated by a GDSL lipase-like enzyme designated as BnSCE3, one of three detected SCE isoforms | Brassica napus |
physiological function | sinapoylmalate is involved in protecting the leaves against the deleterious effects of UV-B radiation. Sinapine might function as storage vehicle for ready supply of choline for phosphatidylcholine biosynthesis in young seedlings | Arabidopsis thaliana |
physiological function | sinapoylmalate is involved in protecting the leaves against the deleterious effects of UV-B radiation. Sinapine might function as storage vehicle for ready supply of choline for phosphatidylcholine biosynthesis in young seedlings. Antinutritive character of sinapine and related sinapate esters | Brassica napus |