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Literature summary for 3.1.1.47 extracted from

  • Stafforini, D.M.; Rollins, E.N.; Prescott, S.M.; McIntyre, T.M.
    The platelet-activating factor acetylhydrolase from human erythrocytes. Purification and properties (1993), J. Biol. Chem., 268, 3857-3865.
    View publication on PubMed
Search for more literature for 3.1.1.47

General Stability

General Stability Organism
highly sensitive to proteolysis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Homo sapiens
diethyldicarbonate
-
 Homo sapiens
dithioerythritol
-
 Homo sapiens
NaF
-
 Homo sapiens
p-bromophenacyl bromide
-
 Homo sapiens
p-nitroguanidinobenzoate
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no stimulation by Ca2+ or Mg2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000 + x * 31000, the 25000 Da component represents the catalytic unit, SDS-PAGE Homo sapiens
31000
-
 x * 25000 + x * 31000, the 25000 Da component represents the catalytic unit, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O Homo sapiens i.e. PAF ?
-
 ?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O Homo sapiens the substrate mediates a wide spectrum of actions, including hypotension, changes in smooth muscle tone, vascular permeability, signalling in inflammation and allergy. The intracelluar form of the enzyme regulates the accumulation of the substrate, e.g. in macrophages and platelets ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from erythrocytes Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.805
-
 purified from erythrocytes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O i.e. PAF Homo sapiens 1-O-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O i.e. PAF Homo sapiens ?
-
 ?
1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O the substrate mediates a wide spectrum of actions, including hypotension, changes in smooth muscle tone, vascular permeability, signalling in inflammation and allergy. The intracelluar form of the enzyme regulates the accumulation of the substrate, e.g. in macrophages and platelets Homo sapiens ?
-
 ?
1-palmitoyl-2-oxopentanoyl-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 1-palmitoyl-sn-glycero-3-phosphocholine + 2-oxopentanoate
-
 ?
additional information the erythrocyte enzyme has a marked selectivity for short-chain acyl chains at sn-2 position, the linkage at the sn-1 postion may be of the ester or ether type and does not influence the catalytic effeciency Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 25000 + x * 31000, the 25000 Da component represents the catalytic unit, SDS-PAGE Homo sapiens