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Literature summary for 3.1.1.47 extracted from

  • Chroni, A.; Mavri-Vavayanni, M.
    Characterization of a platelet activating factor acetylhydrolase from rat adipocyte (2000), Life Sci., 67, 2807-2825.
    View publication on PubMed
Search for more literature for 3.1.1.47

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol slightly acivating Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 18% inhibition at 10 mM Rattus norvegicus
DTNB 69% inhibition at 2 mM Rattus norvegicus
lyso-2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine 22% inhibition at 0.05 mM Rattus norvegicus
additional information no inhibition by EGTA at 10 mM and acetylsalicylic acid at 0.2 mM Rattus norvegicus
phosphocholine 25% inhibition at 0.05 mM Rattus norvegicus
PMSF 93% inhibition at 2 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0049
-
 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine pH 7.2, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol adipocyte Rattus norvegicus 5829
-
additional information activity in subcellular fractions Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 66000, major part, + x * 37000, mino part, SDS-PAGE Rattus norvegicus
66000
-
 x * 66000, major part, + x * 37000, mino part, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine + H2O Rattus norvegicus i.e. platelet-activation factor, substrate and enzyme play important roles in inflammation processes 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 male Wistar rats
-

Purification (Commentary)

Purification (Comment) Organism
from adipocyte, 45.9fold Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
adipocyte
-
 Rattus norvegicus
-
epididymis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 partially purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O
-
 Rattus norvegicus 1-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor Rattus norvegicus 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?
2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor, substrate and enzyme play important roles in inflammation processes Rattus norvegicus 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, major part, + x * 37000, mino part, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
PAF-AH
-
 Rattus norvegicus
platelet activating factor acetylhydrolase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 30 min, stable Rattus norvegicus
60
-
 10 min, almost complete inactivation Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 broad optimum Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5 7.4 inactivation below pH 5.0, active to mild alkaline range Rattus norvegicus