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Literature summary for 3.1.1.47 extracted from
- Platelet-activating factor (PAF)-dependent transacetylase and its relationship with PAF acetylhydrolases (2000), J. Biol. Chem., 275, 26704-26709.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PAF-AH isozyme II, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Escherichia coli, expression of isozyme II mutant enzymes in CHO-K1 cells | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C120S | site-directed mutagenesis, residue is involved in transacetylase reaction mechanism, mutant shows 35% reduced transacetylase activity but unaltered acetylhydrolase activity | Rattus norvegicus |
| G2A | site-directed mutagenesis, residue is involved in transacetylase reaction mechanism, mutant shows 50% reduced transacetylase activity but unaltered acetylhydrolase activity | Rattus norvegicus |
| G2A/C120S | site-directed mutagenesis, residues are involved in transacetylase reaction mechanism, mutant shows 80% reduced transacetylase activity but unaltered acetylhydrolase activity | Rattus norvegicus |
| S234C | site-directed mutagenesis, inactive mutant | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | platelet-activating factor-dependent transacetylase | Rattus norvegicus | 5829 | - |
| membrane | platelet-activating factor-dependent transacetylase | Rattus norvegicus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, cytosolic and membraneous PAF-TA, SDS-PAGE, x * 43491, PAF-AH isozyme II, amino acid sequence calculation | Rattus norvegicus |
| 43491 | - |
x * 40000, cytosolic and membraneous PAF-TA, SDS-PAGE, x * 43491, PAF-AH isozyme II, amino acid sequence calculation | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
2 isozymes II and Ib of platelet-activating factor acetylhydrolase PAF-AH | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PAF-transacetylase from kidney cytosol, to homogeneity, recombinant PAF-AH isozyme II from Escherichia coli | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate | isozyme II of the enzyme also performs platelet-activating factor-dependent transacetylation, transfer of an acetyl group from platelet-activating factor to endogenous acceptor lipids | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | platelet-activating factor-dependent transacetylase | Rattus norvegicus | - |
| lung | platelet-activating factor acetylhydrolase, isozymes II and Ib with alpha1 or alpha2 subunit | Rattus norvegicus | - |
| plasma | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
PAF-AH isozyme Ib alpha1 subunit shows no transacetylase activity | Rattus norvegicus |
| 0.000012 | - |
PAF-AH isozyme Ib alpha2 subunit, transacetylase activity | Rattus norvegicus |
| 0.00028 | - |
PAF-AH isozyme Ib alpha1 subunit, acetylhydrolase activity | Rattus norvegicus |
| 0.00236 | - |
PAF-AH isozyme Ib alpha2 subunit, acetylhydrolase activity | Rattus norvegicus |
| 0.0408 | - |
PAF-AH isozyme II, transacetylase activity | Rattus norvegicus |
| 0.105 | - |
PAF-AH isozyme II, acetylhydrolase activity | Rattus norvegicus |
| 0.114 | - |
plasma PAF-AH, transacetylase activity | Rattus norvegicus |
| 0.131 | - |
plasma PAF-AH, acetylhydrolase activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Rattus norvegicus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, cytosolic and membraneous PAF-TA, SDS-PAGE, x * 43491, PAF-AH isozyme II, amino acid sequence calculation | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | platelet-activating factor-dependent transacetylase, PAF-TA, and platelet-activating factor acetylhydrolase isozyme II, PAF-AH II, might be the same enzyme | Rattus norvegicus |
| PAF-AH | - |
Rattus norvegicus |
| platelet-activating factor acetylhydrolase | - |
Rattus norvegicus |
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