Any feedback?
Literature summary for 3.1.1.45 extracted from
- Evolution of chlorocatechol catabolic pathways. Conclusions to be drawn from comparisons of lactone hydrolases (1994), Biodegradation, 5, 301-321.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cupriavidus necator |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | no inactivation | Burkholderia cepacia |  |
| EDTA | Alcaligenes eutrophus 335 and JMP222; Alcaligenes eutrophus JMP134 no inactivation | Cupriavidus necator | |
| EDTA | no inactivation | Pseudomonas knackmussii | |
| EDTA | no inactivation | Pseudomonas putida | |
| EDTA | no inactivation | Rhodococcus opacus | |
| p-chloromercuribenzoate | not inhibitory | Burkholderia cepacia | |
| p-chloromercuribenzoate | - |
Cupriavidus necator | |
| p-chloromercuribenzoate | - |
Pseudomonas knackmussii | |
| p-chloromercuribenzoate | Pseudomonas putida AC866 | Pseudomonas putida | |
| p-chloromercuribenzoate | - |
Rhodococcus opacus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
trans-4-Carboxymethylenebut-2-en-4-olide | - |
Pseudomonas knackmussii | |
| 0.0281 | - |
cis-4-carboxymethylenebut-2-en-4-olide | - |
Rhodococcus opacus | |
| 0.11 | - |
trans-4-Carboxymethylenebut-2-en-4-olide | Alcaligenes eutrophus JMP134, cell-free extract, not with purified enzyme | Cupriavidus necator | |
| 0.14 | - |
cis-4-carboxymethylenebut-2-en-4-olide | Alcaligenes eutrophus JMP134, cell-free extract, not with purified enzyme | Cupriavidus necator | |
| 0.4 | - |
cis-4-carboxymethylenebut-2-en-4-olide | - |
Pseudomonas knackmussii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | Alcaligenes eutrophus 335, Mn2+ dependent | Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25400 | 25800 | predicted from DNA sequences | Pseudomonas putida |
| 25400 | 25800 | predicted from DNA sequences | Cupriavidus necator |
| 25400 | 25800 | predicted from DNA sequences | Pseudomonas knackmussii |
| 25400 | 25800 | Alcaligenes eutrophus JMP134 | Cupriavidus necator |
| 25400 | 25800 | Pseudomonas putida AC866 | Pseudomonas putida |
| 30000 | - |
gel filtration | Rhodococcus opacus |
| 39000 | - |
gel filtration | Burkholderia cepacia |
| 58000 | - |
Alcaligenes eutrophus 335 and JMP222, gel filtration | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carboxymethylenebut-2-en-4-olide + H2O | Pseudomonas knackmussii | the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia cepacia | - |
type II DLH: cis-dienelactone hydrolase | - |
| Burkholderia cepacia | - |
Pseudomonas cepacia | - |
| Cupriavidus necator | - |
Alcaligenes eutrophus JMP134, type III DLH: hydrolyzes both dienelactone isomers | - |
| Cupriavidus necator | - |
Alcaligenes eutrophus 335, type I DLH: trans-dienelactone hydrolyse | - |
| Cupriavidus necator | - |
Alcaligenes eutrophus JMP222, type I DLH: trans-dienelactone hydrolyse | - |
| Pseudomonas knackmussii | - |
dienelactone hydrolases purified from Pseudomonas sp. B13 and from a clone with pAC27, encoding the 3-chlorobenzoate degradative enzymes in Pseudomonas putida AC866 are identical in every discernible respect. The DLH of Pseudomonas sp. B13 and Pseudomonas putida AC866, pAC27, are therefore not differentiated | - |
| Pseudomonas knackmussii | - |
type III DLH: hydrolyzes both dienelactone isomers | - |
| Pseudomonas putida | - |
dienelactone hydrolases purified from Pseudomonas sp. B13 and from a clone with pAC27, encoding the 3-chlorobenzoate degradative enzymes in Pseudomonas putida AC866 are identical in every discernible respect. The DLH of Pseudomonas sp. B13 and Pseudomonas putida AC866, pAC27, are therefore not differentiated | - |
| Pseudomonas putida | - |
AC866, type III DLH: hydrolyzes both dienelactone isomers | - |
| Pseudomonas sp. P51 | - |
- |
- |
| Rhodococcus opacus | - |
1CP, Rhodococcus erythropolis 1CP | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate | similar catalytic triads are found in alpha/beta hydrolase fold enzymes: DLH is the simplest one | Pseudomonas knackmussii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-oxoadipate enol-lactone + H2O | Alcaligenes eutrophus JMP134 not a substrate | Cupriavidus necator | ? | - |
? | |
| 3-oxoadipate enol-lactone + H2O | Alcaligenes eutrophus 335 and JMP222 | Cupriavidus necator | ? | - |
? | |
| 4-carboxymethylenebut-2-en-4-olide + H2O | the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds | Pseudomonas knackmussii | ? | - |
? | |
| 4-fluoro-muconolactone + H2O | - |
Cupriavidus necator | ? | - |
? | |
| 4-fluoro-muconolactone + H2O | - |
Pseudomonas knackmussii | ? | - |
? | |
| 4-fluoro-muconolactone + H2O | AC866 | Pseudomonas putida | ? | - |
? | |
| cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Burkholderia cepacia | 4-oxohex-2-enedioate | - |
? | |
| cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Rhodococcus opacus | 4-oxohex-2-enedioate | - |
? | |
| cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? | |
| cis-4-carboxymethylenebut-2-en-4-olide + H2O | Alcaligenes eutrophus JPM134 | Cupriavidus necator | 4-oxohex-2-enedioate | - |
? | |
| cis-4-carboxymethylenebut-2-en-4-olide + H2O | Pseudomonas putida AC866 | Pseudomonas putida | 4-oxohex-2-enedioate | - |
? | |
| cis-4-carboxymethylenebut-2-en-4-olide + H2O | Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222: not a substrate to a significant extent | Cupriavidus necator | 4-oxohex-2-enedioate | - |
? | |
| additional information | - |
Rhodococcus opacus | ? | - |
? | |
| additional information | substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones | Cupriavidus necator | ? | - |
? | |
| additional information | substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones | Burkholderia cepacia | ? | - |
? | |
| additional information | substrate specificities differentiate among three types of this activity. Type I: extracts of Alcaligenes eutrophus 335, Alcaligenes eutrophus H16, Alcaligenes eutrophus JMP222 and Alcaligenes strain A7 convert trans-4-carboxymethylenebut-2-en-4-olide, trans-dienelactone, much faster than cis-isomer, type II: enzyme of Pseudomonas cepacia converts cis-dienelactone much faster, type III: enzyme of Alcaligenes eutrophus JMP134 and Pseudomonas strain B13 hydrolyze both dienelactones | Pseudomonas knackmussii | ? | - |
? | |
| trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas putida | 4-oxohex-2-enedioate | - |
? | |
| trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Cupriavidus necator | 4-oxohex-2-enedioate | - |
? | |
| trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? | |
| trans-4-carboxymethylenebut-2-en-4-olide + H2O | not a substrate to a significant extent | Burkholderia cepacia | 4-oxohex-2-enedioate | - |
? | |
| trans-4-carboxymethylenebut-2-en-4-olide + H2O | not a substrate to a significant extent | Rhodococcus opacus | 4-oxohex-2-enedioate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Pseudomonas putida |
| monomer | 1 * 30000, SDS-PAGE | Rhodococcus opacus |
| monomer | alpha,beta protein | Pseudomonas knackmussii |
| monomer | 1* 32400, SDS-PAGE | Burkholderia cepacia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Burkholderia cepacia |
| 7.5 | - |
- |
Cupriavidus necator |
| 7.5 | - |
- |
Pseudomonas knackmussii |
| 7.5 | - |
Pseudomonas putida AC866 | Pseudomonas putida |
| 7.8 | 8 | - |
Rhodococcus opacus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
