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Literature summary for 3.1.1.45 extracted from
- X-ray crystallographic structure of dienelactone hydrolase at 2.8 A (1988), J. Mol. Biol., 204, 435-445.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| C60S DLH | Pseudomonas knackmussii |
| x-ray crystallographic structure at 2.8 A resolution | Pseudomonas knackmussii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C60S | no reduction in activity | Pseudomonas knackmussii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carboxymethylenebut-2-en-4-olide + H2O | Pseudomonas knackmussii | the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas knackmussii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| C60S DLH | Pseudomonas knackmussii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate | the active-site Cys123 residue is part of a triad of residues consisting of Cys123, His202 and Asp171, and is reminiscent of the serine/cysteine proteases | Pseudomonas knackmussii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carboxymethylenebut-2-en-4-olide + H2O | the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds | Pseudomonas knackmussii | ? | - |
? | |
| cis-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? | |
| trans-4-carboxymethylenebut-2-en-4-olide + H2O | - |
Pseudomonas knackmussii | 4-oxohex-2-enedioate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | alpha,beta protein | Pseudomonas knackmussii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DLHase | - |
Pseudomonas knackmussii |
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