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Literature summary for 3.1.1.45 extracted from

  • Frantz, B.; Ngai, K.L.; Chatterjee, D.K.; Ornston, L.N.; Chakrabarty, A.M.
    Nucleotide sequence and expression of clcD, a plasmid-borne dienelactone hydrolase gene from Pseudomonas sp. strain B13 (1987), J. Bacteriol., 169, 704-709.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.45

Cloned(Commentary)

Cloned (Comment) Organism
AC866 expression in Escherichia coli and Pseudomonas putida Pseudomonas putida
expression in Escherichia coli and Pseudomonas putida Pseudomonas knackmussii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25490
-
 calculated from nucleotide sequence of the clcD gene product Pseudomonas putida
25490
-
 calculated from nucleotide sequence of the clcD gene product Pseudomonas knackmussii
28000
-
 AC866, 1 * 28000, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas knackmussii the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas knackmussii
-
 dienelactone hydrolases purified from Pseudomonas sp. B13 and from a clone with pAC27, encoding the 3-chlorobenzoate degradative enzymes in Pseudomonas putida AC866 are identical in every discernible respect. The DLH of Pseudomonas sp. B13 and Pseudomonas putida AC866, pAC27, are therefore not differentiated
-
Pseudomonas putida
-
 dienelactone hydrolases purified from Pseudomonas sp. B13 and from a clone with pAC27, encoding the 3-chlorobenzoate degradative enzymes in Pseudomonas putida AC866 are identical in every discernible respect. The DLH of Pseudomonas sp. B13 and Pseudomonas putida AC866, pAC27, are therefore not differentiated
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida
-
 Pseudomonas knackmussii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxymethylenebut-2-en-4-olide + H2O the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds Pseudomonas knackmussii ?
-
 ?
cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas putida 4-oxohex-2-enedioate
-
 ?
cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
additional information muconolactone is not hydrolyzed to a significant extent Pseudomonas knackmussii ?
-
 ?
trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas putida 4-oxohex-2-enedioate
-
 ?
trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?

Subunits

Subunits Comment Organism
monomer 1* 28000, SDS-PAGE with mercaptoethanol Pseudomonas knackmussii
monomer AC866, 1 * 28000, SDS-PAGE Pseudomonas putida