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Literature summary for 3.1.1.45 extracted from

  • Maltseva, O.V.; Solyanikova, I.P.; Golovleva, L.A.; Schlömann, M.; Knackmuss, H.J.
    Dienelactone hydrolase from Rhodococcus erythropolis 1 CP: purification and properties (1994), Arch. Microbiol., 162, 368-374.
No PubMed abstract available
Search for more literature for 3.1.1.45

General Stability

General Stability Organism
(NH4)2SO4, 0.1-0.8 M, prevented the strong decline of activity Rhodococcus opacus

Inhibitors

Inhibitors Comment Organism Structure
EDTA no inactivation Rhodococcus opacus
additional information no inhibition by 4-fluoromuconolactone, 2-methylmuconolactone, 4-methylmuconolactone, 3-oxoadipate enol-lactone Rhodococcus opacus
p-chloromercuribenzoate
-
 Rhodococcus opacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
 cis-4-carboxymethylenebut-2-chloro-2-en-4-olide
-
 Rhodococcus opacus
0.0281
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Rhodococcus opacus
0.31
-
 cis-4-carboxymethylenebut-2-methyl-2-en-4-olide
-
 Rhodococcus opacus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Rhodococcus opacus

Organism

Organism UniProt Comment Textmining
Rhodococcus opacus
-
 1CP, Rhodococcus erythropolis 1CP
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus opacus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30.3
-
-
 Rhodococcus opacus

Storage Stability

Storage Stability Organism
4°C, 50 mM Tris-HCl, pH 7.2, 0.1 mM dithiothreitol, 0.5 M (NH4)2SO4, stable for over 3 months Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-4-carboxymethylenebut-2-chloro-2-en-4-olide + H2O
-
 Rhodococcus opacus ?
-
 ?
cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Rhodococcus opacus 4-oxohex-2-enedioate
-
 ?
cis-4-carboxymethylenebut-2-methyl-2-en-4-olide + H2O
-
 Rhodococcus opacus ?
-
 ?
additional information no activity with 2-methylmuconolactone, 4-methylmuconolactone Rhodococcus opacus ?
-
 ?
trans-4-carboxymethylenebut-2-en-4-olide + H2O not a substrate to a significant extent Rhodococcus opacus 4-oxohex-2-enedioate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Rhodococcus opacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhodococcus opacus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.95
-
 cis-4-carboxymethylenebut-2-methyl-2-en-4-olide
-
 Rhodococcus opacus
15.4
-
 cis-4-carboxymethylenebut-2-en-4-olide
-
 Rhodococcus opacus
19.8
-
 cis-4-carboxymethylenebut-2-chloro-2-en-4-olide
-
 Rhodococcus opacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8
-
 Rhodococcus opacus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
 Rhodococcus opacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 7.2 highest stability at Rhodococcus opacus