Cloned (Comment) | Organism |
---|---|
expression of the enzyme encoded by gene tfdE in a module I of genes tfdC, tfdD, tfdE, and tfdF organized in one plasmid, functional expression in Ralstonia eutropha JMP222 and in Pseudomonas putida KT2442, 2 strains able to grow on 3-chlorobenzoate | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dienelactone + H2O | Cupriavidus necator | enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate | maleylacetate | - |
? | |
dienelactone + H2O | Cupriavidus necator JMP 134-1 | enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate | maleylacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator JMP 134-1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.61 | - |
crude enzyme extract from wild-type strain Ralstonia eutropha JMP134 | Cupriavidus necator |
1.96 | - |
crude enzyme extract from recombinant strain Ralstonia eutropha JMP222 | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-dienelactone + H2O | - |
Cupriavidus necator | maleylacetate | - |
? | |
cis-dienelactone + H2O | - |
Cupriavidus necator JMP 134-1 | maleylacetate | - |
? | |
dienelactone + H2O | enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate | Cupriavidus necator | maleylacetate | - |
? | |
dienelactone + H2O | enzyme is involved in the degradation of chlorocatechols resulting from growth on from 3-chlorobenzoate and 2,4-dichlorophenoxyacetate | Cupriavidus necator JMP 134-1 | maleylacetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dienelactone hydrolase | - |
Cupriavidus necator |
DLH | - |
Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Cupriavidus necator |