Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acetobacter pasteurianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
cephalexin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
1 | - |
ampicillin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
1.1 | - |
D-2-nitro-5-[(phenylglycyl)amino]benzoic acid | 30°C, pH 6.2 | Acetobacter pasteurianus | |
1.7 | - |
cefadroxil | 30°C, pH 6.2 | Acetobacter pasteurianus | |
2.6 | - |
amoxicillin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
7 | - |
D-phenylglycine methyl ester | 30°C, pH 6.2 | Acetobacter pasteurianus | |
13 | - |
D-phenylglycine amide | 30°C, pH 6.2 | Acetobacter pasteurianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000, SDS-PAGE | Acetobacter pasteurianus |
150000 | - |
dynamic light scattering | Acetobacter pasteurianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alpha-aminoacid ester + H2O | Acetobacter pasteurianus | - |
an alpha-amino acid + an alcohol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter pasteurianus | Q8VRK8 | ATCC 9325 | - |
Purification (Comment) | Organism |
---|---|
CM-Sepharose, hydrophobic interaction, gel filtration | Acetobacter pasteurianus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
667 | - |
Cephalexin synthesis | Acetobacter pasteurianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amoxicillin + H2O | - |
Acetobacter pasteurianus | D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid | - |
? | |
ampicillin + H2O | - |
Acetobacter pasteurianus | (R)-2-phenylglycine + 6-aminopenicillanic acid | - |
r | |
an alpha-aminoacid ester + H2O | - |
Acetobacter pasteurianus | an alpha-amino acid + an alcohol | - |
r | |
cefadroxil + H2O | - |
Acetobacter pasteurianus | ? | - |
r | |
cephalexin + H2O | - |
Acetobacter pasteurianus | D-phenylglycine + 7-aminodesacetoxycephalosporanic acid | - |
r | |
D-2-nitro-5-[(phenylglycyl)amino]benzoic acid + H2O | - |
Acetobacter pasteurianus | phenylglycine + p-nitrophenol | - |
r | |
D-2-nitro-5-[(phenylglycyl)amino]benzoic acid + H2O | - |
Acetobacter pasteurianus | ? | - |
? | |
D-phenylglycine amide + H2O | - |
Acetobacter pasteurianus | D-phenylglycine + NH3 | - |
r | |
D-phenylglycine methyl ester + H2O | - |
Acetobacter pasteurianus | D-phenylglycine + methanol | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70000, SDS-PAGE | Acetobacter pasteurianus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
D-2-nitro-5-[(phenylglycyl)amino]benzoic acid | 30°C, pH 6.2 | Acetobacter pasteurianus | |
9.6 | - |
cefadroxil | 30°C, pH 6.2 | Acetobacter pasteurianus | |
10 | - |
amoxicillin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
43 | - |
D-phenylglycine amide | 30°C, pH 6.2 | Acetobacter pasteurianus | |
162 | - |
ampicillin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
347 | - |
cephalexin | 30°C, pH 6.2 | Acetobacter pasteurianus | |
1035 | - |
D-phenylglycine methyl ester | 30°C, pH 6.2 | Acetobacter pasteurianus | |
1040 | - |
D-phenylglycine methyl ester | 30°C, pH 6.2 | Acetobacter pasteurianus |