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Literature summary for 3.1.1.42 extracted from

  • Nieter, A.; Kelle, S.; Linke, D.; Berger, R.G.
    A p-coumaroyl esterase from Rhizoctonia solani with a pronounced chlorogenic acid esterase activity (2017), New Biotechnol., 37, 153-161 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Rhizoctonia solani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0183
-
chlorogenate pH 6.0, 37°C Rhizoctonia solani
0.0358
-
methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani

Organism

Organism UniProt Comment Textmining
Rhizoctonia solani A0A1D0BQV8 cf. EC 3.1.1.73
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorogenate + H2O 83% of the activity with methyl p-coumarate Rhizoctonia solani caffeate + quinate
-
?
methyl p-coumarate + H2O
-
Rhizoctonia solani methanol + p-coumarate
-
?

Synonyms

Synonyms Comment Organism
FAE3
-
Rhizoctonia solani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Rhizoctonia solani

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37
-
more than 80% of maximum activity Rhizoctonia solani
40
-
rapid loss of activity above Rhizoctonia solani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
1 h, 55% residual activity Rhizoctonia solani
40
-
1 h, almost complete loss of activity Rhizoctonia solani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29.3
-
chlorogenate pH 6.0, 37°C Rhizoctonia solani
33.1
-
methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
925
-
methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
1598
-
chlorogenate pH 6.0, 37°C Rhizoctonia solani