Any feedback?
Literature summary for 3.1.1.42 extracted from
- Coffee pulp koji of Aspergillus sojae as stable immobilized catalyst of chlorogenate hydrolase (2008), Appl. Microbiol. Biotechnol., 81, 143-151.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | coffee powder, coffee pulp | Aspergillus sojae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
determined by SDS-PAGE, monomer | Aspergillus sojae |
| 180000 | - |
determined by gel filtration, homodimer | Aspergillus sojae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorogenate + H2O | Aspergillus sojae | - |
caffeate + quinate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus sojae | - |
Aspergillus sojae AKU 3312 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chlorogenate hydrolase is purifried from mycelia of Aspergillus sojae using a DEAE-cellulose, a CM-Sephadex C-50, a hydroxyapatite and a Sephadex G-200 column | Aspergillus sojae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus sojae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.09 | - |
cell-free extract | Aspergillus sojae |
| 0.34 | - |
purification step DEAE-cellulose | Aspergillus sojae |
| 0.67 | - |
purification step CM-Sephadex C-50 | Aspergillus sojae |
| 4.55 | - |
purification step hydroxyapatite | Aspergillus sojae |
| 24.11 | - |
purification step Sephadex G-200 | Aspergillus sojae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chlorogenate + H2O | - |
Aspergillus sojae | caffeate + quinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Aspergillus sojae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CHase | - |
Aspergillus sojae |
| chlorogenate hydrolase | - |
Aspergillus sojae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Aspergillus sojae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity assay | Aspergillus sojae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
