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Literature summary for 3.1.1.41 extracted from
- The structural basis for the narrow substrate specificity of an acetyl esterase from Thermotoga maritima (2012), Biochim. Biophys. Acta, 1824, 1024-1030 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Thermotoga maritima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S188A | active site knockout | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00041 | - |
fluorescein dibutyloxymethyl ether | pH 7.3, 25°C | Thermotoga maritima |  |
| 0.00051 | - |
((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) | pH 7.3, 25°C | Thermotoga maritima | |
| 0.00054 | - |
((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) | pH 7.3, 25°C | Thermotoga maritima | |
| 0.00096 | - |
((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate | pH 7.3, 25°C | Thermotoga maritima | |
| 0.00097 | - |
fluorescein dipropanoyloxymethyl ether | pH 7.3, 25°C | Thermotoga maritima | |
| 0.001 | - |
fluorescein divaleryloxymethyl ether | pH 7.3, 25°C | Thermotoga maritima | |
| 0.0026 | - |
fluorescein dicaproyloxymethyl ether | pH 7.3, 25°C | Thermotoga maritima | |
| 0.0037 | - |
fluorescein di(acetoxymethyl ether) | pH 7.3, 25°C | Thermotoga maritima | |
| 0.76 | - |
4-nitrophenyl acetate | pH 7.3, 25°C | Thermotoga maritima | |
| 20.8 | - |
7-aminocephalosporanic acid | pH 7.3, 25°C | Thermotoga maritima | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | Q9WXT2 | - |
- |
| Thermotoga maritima DSM 3109 | Q9WXT2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) + H2O | - |
Thermotoga maritima | ? | - |
? | |
| ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) + H2O | - |
Thermotoga maritima DSM 3109 | ? | - |
? | |
| ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) + H2O | - |
Thermotoga maritima | ? | - |
? | |
| ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) + H2O | - |
Thermotoga maritima DSM 3109 | ? | - |
? | |
| ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate + H2O | - |
Thermotoga maritima | ? | - |
? | |
| ((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate + H2O | - |
Thermotoga maritima DSM 3109 | ? | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Thermotoga maritima | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl acetate + H2O | - |
Thermotoga maritima DSM 3109 | 4-nitrophenol + acetate | - |
? | |
| 7-aminocephalosporanic acid + H2O | - |
Thermotoga maritima | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
| 7-aminocephalosporanic acid + H2O | - |
Thermotoga maritima DSM 3109 | acetate + deacetyl-7-aminocephalosporanic acid | - |
? | |
| fluorescein di(acetoxymethyl ether) + H2O | - |
Thermotoga maritima | ? | - |
? | |
| fluorescein dibutyloxymethyl ether + H2O | - |
Thermotoga maritima | ? | - |
? | |
| fluorescein dicaproyloxymethyl ether + H2O | - |
Thermotoga maritima | ? | - |
? | |
| fluorescein dipropanoyloxymethyl ether + H2O | - |
Thermotoga maritima | ? | - |
? | |
| fluorescein divaleryloxymethyl ether + H2O | - |
Thermotoga maritima | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TM0077 | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
assay at | Thermotoga maritima |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
fluorescein divaleryloxymethyl ether | pH 7.3, 25°C | Thermotoga maritima | |
| 0.0046 | - |
((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(1-methylcyclopropane-1-carboxylate) | pH 7.3, 25°C | Thermotoga maritima | |
| 0.015 | - |
fluorescein dibutyloxymethyl ether | pH 7.3, 25°C | Thermotoga maritima | |
| 0.023 | - |
((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) dicyclobutanecarboxylate | pH 7.3, 25°C | Thermotoga maritima | |
| 0.14 | - |
((3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl)bis(oxy))bis(methylene) bis(2-methylacrylate) | pH 7.3, 25°C | Thermotoga maritima | |
| 0.14 | - |
fluorescein dicaproyloxymethyl ether | pH 7.3, 25°C | Thermotoga maritima | |
| 0.83 | - |
7-aminocephalosporanic acid | pH 7.3, 25°C | Thermotoga maritima | |
| 6.4 | - |
fluorescein dipropanoyloxymethyl ether | pH 7.3, 25°C | Thermotoga maritima | |
| 14.1 | - |
fluorescein di(acetoxymethyl ether) | pH 7.3, 25°C | Thermotoga maritima | |
| 23 | - |
4-nitrophenyl acetate | pH 7.3, 25°C | Thermotoga maritima | |
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