BRENDA - Enzyme Database show
show all sequences of 3.1.1.41

A colorimetric assay for the determination of acetyl xylan esterase or cephalosporin C acetyl esterase activities using 7-amino cephalosporanic acid, cephalosporin C, or acetylated xylan as substrate

Martinez-Martinez, I.; Montoro-Garcia, S.; Lozada-Ramirez, J.D.; Sanchez-Ferrer, A.; Garcia-Carmona, F.; Anal. Biochem. 369, 210-217 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
enzyme reaction products deacetyl cephalosporins are a highly valuable starting material for producing semisynthetic beta-lactam antibiotics
Bacillus pumilus
Cloned(Commentary)
Commentary
Organism
gene axe, expression of His-tagged enzyme in Escherichia coli
Bacillus pumilus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Bacillus pumilus
215
-
cephalosporin C
pH 7.3, 25°C, recombinant enzyme
Bacillus pumilus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus pumilus
-
gene axe
-
Bacillus pumilus CECT 5072
-
gene axe
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Bacillus pumilus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-aminocephalosporanic acid + H2O
colorimetric assay method development using bromothymol blue, validation, overview
677538
Bacillus pumilus
7-amino-3-deacetylcephalosporanic acid + acetate
-
-
-
?
7-aminocephalosporanic acid + H2O
colorimetric assay method development using bromothymol blue, validation, overview
677538
Bacillus pumilus CECT 5072
7-amino-3-deacetylcephalosporanic acid + acetate
-
-
-
?
cephalosporin C + H2O
colorimetric assay method development using bromothymol blue, validation, overview
677538
Bacillus pumilus
deacetylcephalosporin C + acetate
-
-
-
?
cephalosporin C + H2O
colorimetric assay method development using bromothymol blue, validation, overview
677538
Bacillus pumilus CECT 5072
deacetylcephalosporin C + acetate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Bacillus pumilus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
enzymatic hydrolysis around pH 7.0 is the most effective method for preparing deacetyl derivatives of cephalosporin
Bacillus pumilus
Application (protein specific)
Application
Commentary
Organism
synthesis
enzyme reaction products deacetyl cephalosporins are a highly valuable starting material for producing semisynthetic beta-lactam antibiotics
Bacillus pumilus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene axe, expression of His-tagged enzyme in Escherichia coli
Bacillus pumilus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetics
Bacillus pumilus
215
-
cephalosporin C
pH 7.3, 25°C, recombinant enzyme
Bacillus pumilus
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
Bacillus pumilus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-aminocephalosporanic acid + H2O
colorimetric assay method development using bromothymol blue, validation, overview
677538
Bacillus pumilus
7-amino-3-deacetylcephalosporanic acid + acetate
-
-
-
?
7-aminocephalosporanic acid + H2O
colorimetric assay method development using bromothymol blue, validation, overview
677538
Bacillus pumilus CECT 5072
7-amino-3-deacetylcephalosporanic acid + acetate
-
-
-
?
cephalosporin C + H2O
colorimetric assay method development using bromothymol blue, validation, overview
677538
Bacillus pumilus
deacetylcephalosporin C + acetate
-
-
-
?
cephalosporin C + H2O
colorimetric assay method development using bromothymol blue, validation, overview
677538
Bacillus pumilus CECT 5072
deacetylcephalosporin C + acetate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Bacillus pumilus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
enzymatic hydrolysis around pH 7.0 is the most effective method for preparing deacetyl derivatives of cephalosporin
Bacillus pumilus
Other publictions for EC 3.1.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749261
Singh
Structural role of a conserve ...
Thermotoga maritima, Thermotoga maritima ATCC 43589, Thermotoga maritima DSM 3109
Proteins
85
694-708
2017
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1
1
2
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18
-
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2
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6
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1
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13
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23
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3
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1
20
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1
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18
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2
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23
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3
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1
20
1
1
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19
19
746975
Singh
Crystal structure of Thermoto ...
Thermotoga maritima, Thermotoga maritima ATCC 43589, Thermotoga maritima DSM 3109
Biochem. Biophys. Res. Commun.
476
63-68
2016
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1
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8
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16
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16
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749834
Ma
-
High-level expression of Ceph ...
Bacillus subtilis, Bacillus subtilis SIL3
Biochem. Eng. J.
114
183-190
2016
-
1
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749837
Ma
-
One-pot enzymatic production ...
Pseudomonas sp. SE83
Biochem. Eng. J.
95
1-8
2015
-
1
-
-
-
-
-
-
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-
-
-
-
1
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1
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752071
Tao
-
Efficient production of perac ...
Bacillus subtilis
Process Biochem.
50
2121-2127
2015
-
-
1
-
1
-
1
5
-
-
-
-
-
1
-
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2
1
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1
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1
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1
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5
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2
1
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-
5
5
729073
Tian
A novel cephalosporin deacetyl ...
Bacillus subtilis, Bacillus subtilis CICC 20034
Appl. Microbiol. Biotechnol.
98
2081-2089
2014
-
-
1
-
-
-
15
-
-
-
2
2
-
2
-
-
1
-
-
-
6
-
13
1
1
1
3
-
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
15
-
-
-
-
2
2
-
-
-
1
-
-
6
-
13
1
1
1
3
-
1
1
1
-
-
1
1
-
-
-
730072
Wang
Double knockout of beta-lactam ...
Escherichia coli, Escherichia coli JM105
J. Biosci. Bioeng.
113
737-741
2012
-
-
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1
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2
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7
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2
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1
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2
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730907
Levisson
Functional and structural char ...
Thermotoga maritima
Proteins
80
1545-1559
2012
-
-
1
1
-
-
2
-
1
2
-
2
-
1
-
-
1
1
-
-
-
-
4
1
1
-
-
-
1
-
-
-
1
-
-
-
-
1
-
1
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-
-
2
1
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1
2
-
2
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1
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-
-
-
4
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
747164
Hedge
The structural basis for the ...
Thermotoga maritima, Thermotoga maritima DSM 3109
Biochim. Biophys. Acta
1824
1024-1030
2012
-
-
1
-
1
-
-
10
-
-
-
-
-
4
-
-
1
-
-
-
-
-
15
-
1
-
-
-
1
-
-
-
-
-
-
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1
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-
1
-
-
-
-
10
-
-
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-
-
-
-
1
-
-
-
-
15
-
1
-
-
-
1
-
-
-
-
-
-
-
10
10
677538
Martinez-Martinez
A colorimetric assay for the d ...
Bacillus pumilus, Bacillus pumilus CECT 5072
Anal. Biochem.
369
210-217
2007
-
1
1
-
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
671502
Takimoto
Batch production of deacetyl 7 ...
Bacillus subtilis, Bacillus subtilis SHS0133
Appl. Microbiol. Biotechnol.
65
263-267
2004
-
1
1
-
-
1
1
-
-
-
-
2
-
5
-
-
1
-
-
-
-
1
4
-
-
-
1
-
-
-
2
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
1
4
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
675216
Basch
Expression of a cephalosporin ...
Rhodotorula toruloides
J. Ind. Microbiol. Biotechnol.
31
531-539
2004
-
1
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
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-
-
-
-
-
-
-
-
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-
-
1
1
-
-
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-
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1
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
652917
Vincent
Multifunctional xylooligosacch ...
Bacillus subtilis
J. Mol. Biol.
330
593-606
2003
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
5
1
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-
-
-
-
-
-
-
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-
-
1
-
-
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-
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-
-
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-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649401
Velasco
Cloning and characterization o ...
Acremonium chrysogenum, Acremonium chrysogenum C10 / ATCC 48272
Appl. Microbiol. Biotechnol.
57
350-356
2001
-
-
-
-
-
-
2
1
1
-
2
-
-
7
-
1
1
-
-
-
-
-
4
-
1
1
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
1
1
-
2
-
-
-
1
1
-
-
-
-
4
-
1
1
-
-
1
1
-
1
-
-
-
-
-
-
651823
Lee
-
Isolation, analysis, and expre ...
Staphylococcus sp.
J. Biochem. Mol. Biol.
34
274-277
2001
-
-
1
-
-
-
-
-
-
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1
-
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1
-
1
1
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2
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1
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1
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1
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1
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1
1
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-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
649397
Cardoza
A cephalosporin C acetylhydrol ...
Amycolatopsis lactamdurans, Amycolatopsis lactamdurans MA4213
Appl. Microbiol. Biotechnol.
54
406-412
2000
-
-
-
-
-
-
5
2
1
-
1
-
-
4
-
-
1
-
-
1
1
-
8
-
-
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
5
-
2
1
-
1
-
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1
-
1
1
-
8
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
170677
Takimoto
High-level expression, purific ...
Bacillus subtilis, Bacillus subtilis SHS 0133
J. Biosci. Bioeng.
87
456-462
1999
-
-
1
-
-
-
-
5
-
-
1
-
-
7
-
-
1
-
-
-
1
1
11
1
1
-
-
-
1
-
-
-
-
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1
-
-
-
-
-
-
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5
-
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1
-
-
-
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1
-
-
1
1
11
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
170672
Sakai
-
Purification and properties of ...
Rhodotorula glutinis, Rhodotorula glutinis 38B1
J. Ferment. Bioeng.
85
53-57
1998
-
-
-
-
-
-
6
4
1
-
2
-
-
2
-
1
1
-
-
-
1
-
8
1
1
1
1
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
4
1
-
2
-
-
-
1
1
-
-
1
-
8
1
1
1
1
4
1
1
-
-
-
-
-
-
-
-
170675
Politino
Purification and characterizat ...
Bacillus subtilis, Rhodotorula toruloides
Appl. Environ. Microbiol.
63
4807-4811
1997
-
-
-
-
-
1
3
1
-
-
2
-
-
2
-
1
1
-
-
-
1
-
7
1
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
1
-
-
2
-
-
-
1
1
-
-
1
-
7
1
1
-
2
-
-
1
-
-
-
-
-
-
-
-
170676
Sakai
Bioconversion of 7-aminocephal ...
Rhodotorula glutinis
Appl. Environ. Microbiol.
62
2669-2672
1996
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
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-
-
-
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-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170674
Mitsushima
Gene cloning, nucleotide seque ...
Bacillus subtilis, Bacillus subtilis SHS 0133
Appl. Environ. Microbiol.
61
2224-2229
1995
-
-
1
-
-
-
-
-
-
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-
8
-
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-
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1
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-
-
-
-
170673
Takimoto
-
Purification, characterization ...
Bacillus subtilis, Bacillus subtilis ATCC 6633, Bacillus subtilis SHS 0133
J. Ferment. Bioeng.
77
17-22
1994
-
-
-
-
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7
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4
2
2
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7
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7
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1
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16
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Abbott
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