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Literature summary for 3.1.1.4 extracted from

  • Zhang, A.; Veesenmeyer, J.L.; Hauser, A.R.
    Phosphatidylinositol 4,5-bisphosphate-dependent oligomerization of the Pseudomonas aeruginosa cytotoxin ExoU (2018), Infect. Immun., 86, e00402 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.4

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylinositol 4,5-bisphosphate presence of phosphatidylinositol 4,5-bisphosphate induces the oligomerization of ExoU, and oligomerization does not require ubiquitin Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
A384G complete loss of cytotoxicity Pseudomonas aeruginosa
E464D 54% of wild-type cytotoxicity Pseudomonas aeruginosa
I609N mutant is relatively insensitive to activation by ubiquitin, but with excess ubiquitin it is synergistically activated by phosphatidylinositol 4,5-bisphosphate Pseudomonas aeruginosa
K359N 77% of wild-type cytotoxicity Pseudomonas aeruginosa
K395R 110% of wild-type cytotoxicity Pseudomonas aeruginosa
additional information combining inactive truncated ExoU proteins partially restores phospholipase activity and cytotoxicity Pseudomonas aeruginosa
N369D 110% of wild-type cytotoxicity Pseudomonas aeruginosa
S142A catalytically inactive Pseudomonas aeruginosa
V423F 50% of wild-type cytotoxicity Pseudomonas aeruginosa
V462F 37% of wild-type cytotoxicity Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane localizes to the inner leaflet of the plasma membrane following injection into mammalian cells Pseudomonas aeruginosa 16020
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 gel filtration and multiangle light scattering Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa O34208
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-

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Pseudomonas aeruginosa
More upon injection in HeLa cells, ExoU self-associates and forms complexes. Presence of phosphatidylinositol 4,5-bisphosphate is required for oligomerization Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
ExoU
-
 Pseudomonas aeruginosa