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Literature summary for 3.1.1.4 extracted from

  • Kotani, S.; Izawa, S.; Komai, N.; Takayanagi, A.; Arioka, M.
    Mitochondria-localized phospholipase A2, AoPlaA, in Aspergillus oryzae displays phosphatidylethanolamine-specific activity and is involved in the maintenance of mitochondrial phospholipid composition (2016), Fungal Genet. Biol., 96, 1-11 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
R238A the activity of the R238A mutant isx0140% of that of wild type enzyme. The growth of the strain overexpressing R238A is similar to that of the strain overexpressing wild type AoPlaA, presumably because the residual activity of R238A is sufficient to cause growth retardation Aspergillus oryzae
S266A mutation abolishes the enzyme activity Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Aspergillus oryzae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ free Ca2+ enhances the activity of mAoPlaA, but even without the added Ca2+ or in the presence of EGTA to chelate free Ca2+, mAoPlaA displays more than 20% of maximum activity Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae C7G4B6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-palmitoyl-2-oleoyl-phosphatidylethanolamine + H2O phosphatidylethanolamine-specific activity. The enzyme displays phospholipases A2 activity to phosphatidylethanolamine, but not to other phospholipids and generates 1-acylated lyso-phosphatidylethanolamine Aspergillus oryzae 1-palmitoyl-lysophosphatidylethanolamine + oleate
-
?

Synonyms

Synonyms Comment Organism
mAoPlaA
-
Aspergillus oryzae

General Information

General Information Comment Organism
malfunction the AoplaA-disruption strain displays increased content of cardiolipin Aspergillus oryzae
metabolism the enzyme is involved in the maintenance of mitochondrial phospholipid composition Aspergillus oryzae