Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.4 extracted from

  • Feng, V.; Joh, Y.; Ishikawa, K.; Ishida, H.; Ando, S.; Yamagaki, T.; Nakanishi, H.; Cao, S.; Matsui, I.; Kosugi, Y.
    Thermophilic phospholipase A2 in the cytosolic fraction from the archaeon Pyrococcus horikoshii (2000), J. Am. Oil Chem. Soc., 77, 1147-1152 .
No PubMed abstract available
Search for more literature for 3.1.1.4

Activating Compound

Activating Compound Comment Organism Structure
glycerol 3-20% (v/v), activity is enhanced more than 5-18fold Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ activity is unaffected by the addition of 0.0001-1 mM calcium, but is inhibited slightly by the addition of 2-10 mM calcium Pyrococcus horikoshii
additional information activity is unaffected by the addition of 1-5 mM EDTA or 0.01-20 mM dithiothreitol Pyrococcus horikoshii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Pyrococcus horikoshii 5829
-

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-
Pyrococcus horikoshii OT-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, the phospholipase A2 is difficult to purify by general chromatography because of aggregation Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hexadecanoyl-2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminohexanoyl]-sn-glycero-3-phosphocholine + H2O sensitive fluorescent substrate that is stable at 95°C Pyrococcus horikoshii 1-hexadecanoyl-sn-glycero-3-phosphocholine + 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoic acid
-
 ?
1-hexadecanoyl-2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminohexanoyl]-sn-glycero-3-phosphocholine + H2O sensitive fluorescent substrate that is stable at 95°C Pyrococcus horikoshii OT-3 1-hexadecanoyl-sn-glycero-3-phosphocholine + 6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoic acid
-
 ?
1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonate
-
 ?
1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii OT-3 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonate
-
 ?
1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii 1-palmitoyl-sn-glycero-3-phosphoethanolamine + arachidonate
-
 ?
1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii 1-palmitoyl-sn-glycero-3-phosphocholine + linoleate
-
 ?
1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii OT-3 1-palmitoyl-sn-glycero-3-phosphocholine + linoleate
-
 ?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + H2O the enzyme has similar activities toward 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine and 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine Pyrococcus horikoshii OT-3 1-palmitoyl-sn-glycero-3-phosphocholine + oleate
-
 ?
additional information no fatty acid is produced by the cell lysate when trimyristin, tristearin, or triolein is incubated at 90°C overnight. Therefore, no thermostable lipase activity is observed in the PLA2 from Pyrococcus horikoshii Pyrococcus horikoshii ?
-
 ?
additional information no fatty acid is produced by the cell lysate when trimyristin, tristearin, or triolein is incubated at 90°C overnight. Therefore, no thermostable lipase activity is observed in the PLA2 from Pyrococcus horikoshii Pyrococcus horikoshii OT-3 ?
-
 ?

Synonyms

Synonyms Comment Organism
PLA1
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
80 95 80°C: about 80% of maximal activity, 95°C: about 20% of maximal activity Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 the enzyme retains more than 95% activity for 80 min at pH 7.5 Pyrococcus horikoshii
90
-
 the enzyme retains more than 85% activity for 80 min at pH 7.5 Pyrococcus horikoshii
100
-
 80 min, pH 7.5, 65% inactivation Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 pH 6.5: about 50% of maximal acfivity, pH 7.5: 75% of maximal activity Pyrococcus horikoshii