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Literature summary for 3.1.1.4 extracted from
- Purification and characterization of a phospholipase A2-IIA from common stingray (Dasyatis pastinaca) intestine (2013), Indian J. Biochem. Biophys., 50, 186-195.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sodium deoxycholate | or sodium taurodeoxycholate, required, optimum concentration 6 mM | Dasyatis pastinaca |  |
| sodium taurodeoxycholate | or sodium deoxycholate, required, optimum concentration 4 mM | Dasyatis pastinaca | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, complete inhibition | Dasyatis pastinaca | |
| EGTA | 10 mM, complete inhibition | Dasyatis pastinaca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | absolutely required, optimum concentration 8 mM | Dasyatis pastinaca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
PAGE | Dasyatis pastinaca |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-propanol | 50%, 97% of initial activity | Dasyatis pastinaca |
| Acetone | 50%, no changes in activity | Dasyatis pastinaca |
| acetonitrile | 50%, 15% increase in activity | Dasyatis pastinaca |
| Ethanol | 50%, 23% increase in activity | Dasyatis pastinaca |
| Methanol | 50%, no changes in activity | Dasyatis pastinaca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dasyatis pastinaca | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | small amount of N-glycosylation | Dasyatis pastinaca |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Dasyatis pastinaca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | - |
Dasyatis pastinaca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 290 | - |
pH 9.5, 45°C, presence of sodium deoxycholate and CaCl2 | Dasyatis pastinaca |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 120 days, 95% residual activity | Dasyatis pastinaca |
| 4°C, 120 days, 85% residual activity | Dasyatis pastinaca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-stearoyl-2-oleoyl-sn-glycerol-3-phosphocholine + H2O | - |
Dasyatis pastinaca | 1-stearoyl-sn-glycero-3-phosphocholine + oleic acid | hydrolysis occurs only in sn2 position of phospholipids | ? | |
| egg yolk + H2O | - |
Dasyatis pastinaca | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 14000, SDS-PAGE | Dasyatis pastinaca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Dasyatis pastinaca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
20 min, 80% residual activity | Dasyatis pastinaca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Dasyatis pastinaca |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
maintains more than 70% of activity after incubation at pH 2 | Dasyatis pastinaca |
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