Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-bromophenacyl bromide | inhibits both the catalytic and anticoagulant activities. Inhibition of catalytic activity is approximately 6fold higher compared with inhibition of anticoagulant activity | Daboia russelii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
phosphatidylcholine | pH 8.0, 37°C | Daboia russelii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13840 | - |
MALDI-TOF mass spectrometry | Daboia russelii |
14000 | - |
PAGE | Daboia russelii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daboia russelii | - |
subsp. russelii | - |
Purification (Comment) | Organism |
---|---|
- |
Daboia russelii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Daboia russelii | - |
Storage Stability | Organism |
---|---|
-20°C, five cycles of freezing-thawing do not affect the catalytic or anticoagulant activity | Daboia russelii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitro-3-(octanoyloxy) benzoic acid + H2O | - |
Daboia russelii | ? | - |
? | |
phosphatidylcholine + H2O | - |
Daboia russelii | 1-acylglycerophosphocholine + fatty acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1* 13835, MALDI-TOF mass spectrometry, 1 * 14000, SDS-PAGE | Daboia russelii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | - |
Daboia russelii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Daboia russelii |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme shows strong anticoagulant effect via thrombin inhibition with a Ki value of 380 nM as well as by binding to pro-coagulant phospholipids of plasma. In ex-vivo conditionsthe enzyme at 1.0 microM is non-hemolytic and non-cytotoxic to mammalian cells. It does not inhibit the collagen-induced aggregation of platelets. At a dose of 5 mg/kg, the enzyme is not lethal to mice after 48 h of injection | Daboia russelii |