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Literature summary for 3.1.1.4 extracted from

  • Mukherjee, A.K.
    A major phospholipase A2 from Daboia russelii russelii venom shows potent anticoagulant action via thrombin inhibition and binding with plasma phospholipids (2014), Biochimie, 99, 153-161.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
p-bromophenacyl bromide inhibits both the catalytic and anticoagulant activities. Inhibition of catalytic activity is approximately 6fold higher compared with inhibition of anticoagulant activity Daboia russelii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.61
-
phosphatidylcholine pH 8.0, 37°C Daboia russelii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13840
-
MALDI-TOF mass spectrometry Daboia russelii
14000
-
PAGE Daboia russelii

Organism

Organism UniProt Comment Textmining
Daboia russelii
-
subsp. russelii
-

Purification (Commentary)

Purification (Comment) Organism
-
Daboia russelii

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Daboia russelii
-

Storage Stability

Storage Stability Organism
-20°C, five cycles of freezing-thawing do not affect the catalytic or anticoagulant activity Daboia russelii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitro-3-(octanoyloxy) benzoic acid + H2O
-
Daboia russelii ?
-
?
phosphatidylcholine + H2O
-
Daboia russelii 1-acylglycerophosphocholine + fatty acid
-
?

Subunits

Subunits Comment Organism
monomer 1* 13835, MALDI-TOF mass spectrometry, 1 * 14000, SDS-PAGE Daboia russelii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 45
-
Daboia russelii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
Daboia russelii

General Information

General Information Comment Organism
physiological function enzyme shows strong anticoagulant effect via thrombin inhibition with a Ki value of 380 nM as well as by binding to pro-coagulant phospholipids of plasma. In ex-vivo conditionsthe enzyme at 1.0 microM is non-hemolytic and non-cytotoxic to mammalian cells. It does not inhibit the collagen-induced aggregation of platelets. At a dose of 5 mg/kg, the enzyme is not lethal to mice after 48 h of injection Daboia russelii