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Literature summary for 3.1.1.4 extracted from

  • Saikia, D.; Thakur, R.; Mukherjee, A.
    An acidic phospholipase A2 (RVVA-PLA2-I) purified from Daboia russelli venom exerts its anticoagulant activity by enzymatic hydrolysis of plasma phospholipids and by non-enzymatic inhibition of factor Xa in a phospholipids/Ca2+ independent manner (2011), Toxicon, 57, 841-850.
    View publication on PubMed
Search for more literature for 3.1.1.4

Inhibitors

Inhibitors Comment Organism Structure
4-bromophenacyl bromide
-
 Daboia russelii
AIPLAI PLA2 inhibitor isolated from the methanol extract of Azadirachta indica Daboia russelii
dithiothreitol
-
 Daboia russelii
EDTA
-
 Daboia russelii
factor Xa
-
 Daboia russelii
iodoacetamide
-
 Daboia russelii
N-alpha-p-tosyl-L-lysine chloromethyl ketone
-
 Daboia russelii
N-tosyl-L-phenylalanyl chloromethyl ketone
-
 Daboia russelii
phenylmethylsulfonyl fluoride
-
 Daboia russelii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.65
-
 phosphatidylcholine pH 8.0, 37°C Daboia russelii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28500
-
 2 * 28500, SDS-PAGE Daboia russelii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipids + H2O Daboia russelii
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Daboia russelii
-
 Russell's viper
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Daboia russelii

Purification (Commentary)

Purification (Comment) Organism
reverse phase C18-mu-Nova pack column chromatography, CM Sephadex C-50 gel filtration and DEAE Sephadex A-50 gel filtration Daboia russelii

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Daboia russelii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 crude enzyme has a specific activity of 0.00012 units/mg, after 7fold purification the enzyme has a specific activity of 0.00084 units/mg, at pH 8.0 and 37°C, one unit of activity is defined as the amount of protein which produces a decrease in 0.01 absorbance in 10 min at 740 nm Daboia russelii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acidic PLA2 non-inhibits the factor Xa in a non-enzymatic mechanism Daboia russelii ?
-
 ?
additional information the enzyme does not show any proteolytic, ATPase and AchEase activity at a dose of 750 mM Daboia russelii ?
-
 ?
phosphatidylcholine + H2O the enzyme shows preferential hydrolysis of phosphatidylcholine Daboia russelii ?
-
 ?
phospholipids + H2O
-
 Daboia russelii ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 28500, SDS-PAGE Daboia russelii

Synonyms

Synonyms Comment Organism
acidic phospholipase A2
-
 Daboia russelii
RVV acidic PLA2-I
-
 Daboia russelii
RVVA-PLA2-I
-
 Daboia russelii

pH Range

pH Minimum pH Maximum Comment Organism
8 8.5
-
 Daboia russelii

General Information

General Information Comment Organism
physiological function the enzyme inhibits the blood coagulation cascade non-enzymatically by binding with coagulation factor Xa, even in the absence of phospholipids and Ca2+ and thus slows down the blood coagulation by partially inhibiting the prothrombin activation Daboia russelii