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Literature summary for 3.1.1.4 extracted from
- Purification and biochemical characterization of pancreatic phospholipase A2 from the common stingray Dasyatis pastinaca (2011), Lipids Health Dis., 10, 32.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | PLA2 stability in the presence of organic solvents, as well as in acidic and alkaline pH and at high temperature makes it a good candidate for its application in food industry | Dasyatis pastinaca |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 17 | - |
phosphatidylcholine | at pH 8.5 and 40°C | Dasyatis pastinaca |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
1 * 14000, SDS-PAGE | Dasyatis pastinaca |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-propanol | 97% and 93% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
| Acetone | 95% and 92% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
| acetonitrile | 105% activity at 50% (v/v) after 1 h and 2 h incubation | Dasyatis pastinaca |
| Ethanol | 116% and 100% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
| Methanol | 98% and 95% residual activity after 1 h and 2 h, respectively, at 50% (v/v) | Dasyatis pastinaca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dasyatis pastinaca | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Dasyatis pastinaca |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, ethanol fractionation, Mono-S Sepharose column chromatography, Sephadex G-50 gel filtration, RP-HPLC C-8 column chromatography, and Mono-Q column chromatography | Dasyatis pastinaca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Dasyatis pastinaca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 750 | - |
purified enzyme, in the presence of 4 mM sodium taurodeoxycholate and 8 mM CaCl2 using phosphatidylcholine as substrate, at pH 8.0 and 40°C | Dasyatis pastinaca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Dasyatis pastinaca | 1-acylglycerophosphocholine + a carboxylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 14000, SDS-PAGE | Dasyatis pastinaca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pancreatic-type PLA2 | - |
Dasyatis pastinaca |
| PLA2 | - |
Dasyatis pastinaca |
| sPLA2-IB | - |
Dasyatis pastinaca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Dasyatis pastinaca |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 187 | - |
phosphatidylcholine | at pH 8.5 and 40°C | Dasyatis pastinaca | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Dasyatis pastinaca |
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