Any feedback?
Literature summary for 3.1.1.4 extracted from
- Group IVA phospholipase A2 is necessary for the biogenesis of lipid droplets (2008), J. Biol. Chem., 283, 27369-27382.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| CHO-K1 cells or HEK cells stably expressing N-terminal enhanced green fluorescent protein-cPLA2alpha fusion protein | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S505A | is not phosphorylated at Ser505 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arachidonyl trifluoromethyl ketone | AACOCF3, inhibits release of [3H]arachidonic acid by a 0.01 mM concentration | Homo sapiens |  |
| methylarachidonyl fluorophosphonate | inhibits release of [3H]arachidonic acid by a 0.01 mM concentration | Homo sapiens | |
| additional information | bromoenol lactone does not inhibit release of [3H]arachidonic acid by a 0.01 mM concentration | Homo sapiens | |
| pyrrolidine-2 | inhibits release of [3H]AA by a 0.001 mM concentration | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
endogenous enzyme | Homo sapiens |
| 135000 | - |
transfected protein | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryonic kidney cell line | - |
Homo sapiens | - |
| neuroblastoma cell | SH-5YSY cell | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | phosphorylation at Ser505 is key for enzyme activity and lipid droplets formation | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cPLA2alpha | - |
Homo sapiens |
| group IVA cytosolic phospholipase A2 | - |
Homo sapiens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | siRNA1 reduces the expression of cPLA2alpha | down |
| Homo sapiens | fetal bovine serum and serum starved conditions stimulate cPLA2alpha | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inhibition of cPLA2 abolishes release of arachidonate but not palmitate from cells treated with fetal bovine serum. Inhibition of PLA2 precludes fetal bovine serum-stimulated formation of lipid droplets. Silenced expression of cPLA2 inhibits the formation of lipid droplets. Inhibition of cPLA2alpha under lipid droplets forming conditions alters endoplasmic reticulum structure | Homo sapiens |
| physiological function | cPLA2alpha contribution to lipid droplets biogenesis. Overexpression of cPLA2alpha enhances occurrence of lipid droplets. Role in lipid droplets biogenesis is not because of the generation of arachidonic acid, nor is it related to neutral lipid synthesis. cPLA2 is not involved in the synthesis of neutral lipids during lipid droplets biogenesis. cPLA2alpha is not involved in the channeling of extracellular fatty acids into neutral lipids | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
