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Literature summary for 3.1.1.4 extracted from

  • Fernandez, J.; Gutierrez, J.M.; Angulo, Y.; Sanz, L.; Juarez, P.; Calvete, J.J.; Lomonte, B.
    Isolation of an acidic phospholipase A2 from the venom of the snake Bothrops asper of Costa Rica: biochemical and toxicological characterization (2010), Biochimie, 92, 273-283.
    View publication on PubMed
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
cDNA cloned into the pGEM-T vector and transformed into Escherichia coli strain DH5alpha Bothrops asper

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14180
-
 sequence analysis Bothrops asper
14210
-
 electrospray ionization Bothrops asper
15000
-
 1 * 15000, SDS-PAGE, 1 * 16000, SDS-PAGE, both under reducing and non-reducing conditions Bothrops asper
16000
-
 1 * 15000, SDS-PAGE, 1 * 16000, SDS-PAGE, both under reducing and non-reducing conditions Bothrops asper

Organism

Organism UniProt Comment Textmining
Bothrops asper P20474
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Bothrops asper

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
 Bothrops asper
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
egg yolk phospholipid + H2O
-
 Bothrops asper ?
-
 ?
sn-3-phosphatidylcholine + H2O
-
 Bothrops asper ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 15000, SDS-PAGE, 1 * 16000, SDS-PAGE, both under reducing and non-reducing conditions Bothrops asper

Synonyms

Synonyms Comment Organism
phospholipase A2
-
 Bothrops asper
PLA2
-
 Bothrops asper

pI Value

Organism Comment pI Value Maximum pI Value
Bothrops asper 2D electrophoresis
-
 4.9
Bothrops asper sequence analysis
-
 5.05

General Information

General Information Comment Organism
physiological function acidic PLA2-II, in contrast to the basic Asp49 counterparts present in the same venom, lacks myotoxic, cytotoxic, and anticoagulant activities. It does not show hypotensive and anti-platelet aggregation activities and is not lethal to mice at intravenous doses up to 100 microg (5.9 microg/g), indicating its lack of neurotoxic activity. It shows a moderate induction of local edema in vivo. Acidic PLA2-II does not play a key role in the pathophysiology of envenomings, its purpose may be restricted to digestive functions. Acidic and basic PLA2s form two different antigenic groups in venom Bothrops asper