Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozymes sPLA2-X in Spodoptery frugiperda Sf9 cells using the baculovirus transfection system, expression of wild-type and D47K mutant isozymes sPLA2-X in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D47E | site-directed mutagenesis, inactive mutant | Homo sapiens |
D47K | site-directed mutagenesis, inactive mutant | Homo sapiens |
H46N | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y50F | site-directed mutagenesis, inactive mutant | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted PLA2 | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | PLA2s affect cellular activation, signal transduction, and possibly innate immunity, the specific secretory isozyme sPLA2-X neutralizes human immunodeficiency virus type 1 through degradation of the viral membrane, the catalytic function is required for antiviral activity, the target cells of infection are unaffected, sPLA2-X potently reduces gene transfer of HIV-1 Env-pseudo-typed lentivirus vectors and inhibits the replication of both CCR5- and CXCR4-tropic HIV-1 in human CD4 T cells. Virions resistant to damage by antibody and complement were sensitive to lysis by sPLA2-X, mechanism, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
secreted human phospholipase A2, i.e. sPLA2-X | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
sPLA2-X antiviral effect, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dithio-heptanoylphosphatidylcholine + H2O | detection of reaction product with 5,5'-dithio-bis-(2-nitrobenzoic acid) | Homo sapiens | ? | - |
? | |
additional information | PLA2s affect cellular activation, signal transduction, and possibly innate immunity, the specific secretory isozyme sPLA2-X neutralizes human immunodeficiency virus type 1 through degradation of the viral membrane, the catalytic function is required for antiviral activity, the target cells of infection are unaffected, sPLA2-X potently reduces gene transfer of HIV-1 Env-pseudo-typed lentivirus vectors and inhibits the replication of both CCR5- and CXCR4-tropic HIV-1 in human CD4 T cells. Virions resistant to damage by antibody and complement were sensitive to lysis by sPLA2-X, mechanism, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PLA2 | - |
Homo sapiens |
secreted human phospholipase A2 | - |
Homo sapiens |
sPLA2-X | - |
Homo sapiens |