Literature summary for 3.1.1.4 extracted from

Pan, Y.H.; Bahnson, B.J.
Structural basis for bile salt inhibition of pancreatic phospholipase A2 (2007), J. Mol. Biol., 369, 439-450.

Activating Compound

Activating Compound	Comment	Organism
cholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa
glycochenodeoxycholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa
glycocholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa
taurochenodeoxycholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa
taurocholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa

Crystallization (Commentary)

Crystallization (Comment)	Organism
pancreatic PLA2 in complex with five different naturally occurring bile salts, i.e. cholate, taurocholate, glycocholate, glycochenodeoxycholate, and taurochenodeoxycholate, in presence or absence of active-site-directed tetrahedral mimic MJ33, hanging drop vapour diffusion method, 15 mg/ml protein solution with 10 mM CaCl2, 3 mM MJ33, and 3 mM bile salt, is mixed with an equal volume of 0.001 ml of cyrstallization solution containing 0.2 M CaCl2, 0.1 M HEPES, pH 7.5, and 28% v/v PEG 400 or 0.1 M sodium cacodylate, pH 6.5, and 1.4 M sodium acetate, 1 week to 2 months, X-ray diffraction structure determination and analysis at 1.85-2.20 A resolution, modeling	Sus scrofa

Crystallization (Comment)

Organism

pancreatic PLA2 in complex with five different naturally occurring bile salts, i.e. cholate, taurocholate, glycocholate, glycochenodeoxycholate, and taurochenodeoxycholate, in presence or absence of active-site-directed tetrahedral mimic MJ33, hanging drop vapour diffusion method, 15 mg/ml protein solution with 10 mM CaCl2, 3 mM MJ33, and 3 mM bile salt, is mixed with an equal volume of 0.001 ml of cyrstallization solution containing 0.2 M CaCl2, 0.1 M HEPES, pH 7.5, and 28% v/v PEG 400 or 0.1 M sodium cacodylate, pH 6.5, and 1.4 M sodium acetate, 1 week to 2 months, X-ray diffraction structure determination and analysis at 1.85-2.20 A resolution, modeling

Sus scrofa

Inhibitors

Inhibitors	Comment	Organism
cholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa
glycochenodeoxycholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa
glycocholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa
MJ33	i.e. 1-hexadecyl-3-(trifluoroethyl)-rac-glycero-2-phosphomethanol, an active-site-directed tetrahedral mimic	Sus scrofa
taurochenodeoxycholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa
taurocholate	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Sus scrofa	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	P00592	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Sus scrofa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview	Sus scrofa	?	-	?

Synonyms

Synonyms	Comment	Organism
pancreatic phospholipase A2	-	Sus scrofa