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Literature summary for 3.1.1.4 extracted from

  • Hirschberg, H.J.H.B.; Simons, J.W.F.A.; Dekker, N.; Egmond, M.R.
    Cloning, expression, purification and characterization of patatin, a novel phospholipase A (2001), Eur. J. Biochem., 268, 5037-5044.
    View publication on PubMed
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
D192A no enzymatic activity Solanum tuberosum
H175N 10% of wild-type activity Solanum tuberosum
H288N as active as wild-type enzyme Solanum tuberosum
K17A no enzymatic activity Solanum tuberosum
S10C 29% of wild-type activity Solanum tuberosum
S10T 25% of wild-type activity Solanum tuberosum
S234C no enzymatic activity Solanum tuberosum
S234T no enzymatic activity Solanum tuberosum
S54A no enzymatic activity Solanum tuberosum
S54C 1% of wild-type activity Solanum tuberosum
S54T no enzymatic activity Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
methyl-4-nitrophenyl-octylphosphonate inactivates at 100fold molar excess Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 SDS-PAGE Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
 4-nitrophenylcaprate as substrate Solanum tuberosum
109
-
 1,2-dinonanoyl-sn-glycero-3-phosphocholine as substrate Solanum tuberosum
127
-
 1,2-diocytanoyl-sn-glycero-3-phosphocholine as substrate Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dinonanoyl-sn-glycero-3-phosphocholine + H2O
-
 Solanum tuberosum ?
-
 ?
1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O
-
 Solanum tuberosum 1-octanoyl-sn-glycero-3-phosphocholine + octanoate
-
 ?
4-nitrophenyl caprate + H2O
-
 Solanum tuberosum 4-nitrophenol + capric acid
-
 ?
additional information enzyme is active towards mono-acyl-glycerolphosphocholines, increasing activity with increasing chain lengths from C12 to C16 Solanum tuberosum ?
-
 ?

Synonyms

Synonyms Comment Organism
patatin
-
 Solanum tuberosum