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Literature summary for 3.1.1.4 extracted from

  • Lee, J.H.; Ahn, S.H.; Kim, S.H.; Choi, Y.H.; Park, K.J.; Kong, I.S.
    Characterization of Vibrio mimicus phospholipase A (PhlA) and cytotoxicity on fish cell (2002), Biochem. Biophys. Res. Commun., 298, 269-276.
    View publication on PubMed
Search for more literature for 3.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Vibrio mimicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ increases activity by 125% at 1 mM Vibrio mimicus
Co2+ increases activity by 270% at 1 mM, 150% increase at 0.1 mM Vibrio mimicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 SDS-PAGE Vibrio mimicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Vibrio mimicus enzyme has cytotoxic activity and can lyse erythrocytes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio mimicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio mimicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.8
-
-
 Vibrio mimicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-hexadecyl-2-[(cis)-9-octadecenoyl]-rac-glycero-3-phosphocholine + H2O
-
 Vibrio mimicus 1-O-hexadecyl-rac-glycero-3-phosphocholine + cis-9-octadecenoate
-
 ?
1-palmitoyl-2-linoleoylphosphocholine + H2O
-
 Vibrio mimicus 1-palmitoyl-glycerophosphocholine + linolic acid
-
 ?
additional information cleaves fatty acids at the sn-2 and sn-2 positions of phosphatidylcholine Vibrio mimicus ?
-
 ?
additional information enzyme has cytotoxic activity and can lyse erythrocytes Vibrio mimicus ?
-
 ?

Synonyms

Synonyms Comment Organism
PhlA
-
 Vibrio mimicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Vibrio mimicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 relatively stable Vibrio mimicus
50
-
 sharp decrease of activity above Vibrio mimicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Vibrio mimicus