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Literature summary for 3.1.1.4 extracted from
- Properties of purified detergent-resistant phospholipase A of Escherichia coli K-12 (1979), J. Biochem., 86, 1129-1138.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | - |
Escherichia coli |  |
General Stability
| General Stability | Organism |
|---|---|
| detergent-resistant enzyme, Triton X-100 prevents the enzymes thermal inactivation at 37øC | Escherichia coli |
| sodium cholate prevents the enzymes thermal inactivation at 37øC | Escherichia coli |
| sodium deoxycholate prevents the enzymes thermal inactivation at 37øC | Escherichia coli |
| Span 40 prevents the enzymes thermal inactivation at 37C > | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
phosphatidylethanolamine | active enzyme | Escherichia coli | |
| 0.167 | - |
phosphatidylethanolamine | inactivated enzyme | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Escherichia coli | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
SDS-PAGE | Escherichia coli |
| 29000 | - |
SDS-PAGE, enzyme previously inactivated by heat treatment at 80°C or boiled in solution containing SDS | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipids + H2O | Escherichia coli | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel chromatography | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | - |
Escherichia coli | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| phosphatidylethanolamine + H2O | - |
Escherichia coli | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
| phospholipids + H2O | - |
Escherichia coli | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 43 | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | aqueous solution of purified enzyme, with Triton X-100 | Escherichia coli |
| 37 | 70 | aqueous solution of purified enzyme, without Triton X-100 | Escherichia coli |
| 100 | - |
crude preparation, even activation observable | Escherichia coli |
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